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Real-time analysis of platelet aggregation and procoagulant activity during thrombus formation in vivo
http://hdl.handle.net/10271/82
http://hdl.handle.net/10271/8274692857-2099-4f47-a240-fdeb1e1713c2
名前 / ファイル | ライセンス | アクション |
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論文本文 (909.0 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2013-08-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Real-time analysis of platelet aggregation and procoagulant activity during thrombus formation in vivo | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | platelet aggregation, | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | procoagulant activity | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphatidylserine | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | calcium signaling, | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | GFP transgenic mice | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | intravital microscopy | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
その他のタイトル | ||||||
その他のタイトル | 生体内での血栓形成過程における血小板凝集と血栓原性発現のリアルタイム解析 | |||||
言語 | ja | |||||
著者 |
Hayashi, Tadataka
× Hayashi, Tadataka |
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書誌情報 |
en : Pflugers Archiv European Journal of Physiology 巻 456, 号 6, p. 456-456, 発行日 2008-06-14 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Springer | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Copyright Springer | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The exact mechanism of blood vessel thrombus formation remains to be defined. Here, we introduce a new approach to probe thrombus formation in blood vessels of living animals using intravital microscopy in green fluorescent protein (GFP)-transgenic mice to simultaneously monitor platelet aggregation and procoagulant activity. To this end, GFP-expressing platelets and annexin A5 labeled with a fluorescent dye were employed to visualize and analyze platelet aggregation and markers of procoagulant activity (platelet surface phosphatidylserine, PS). Laser-induced thrombi increased and then decreased in size with time in vessels of living animals, whereas platelet surface PS initiated at the site of injury and then penetrated into the thrombus. PS-positive platelets were predominantly localized in the center of the thrombus, as was fibrin generation. The experimental system proposed here is a valuable tool not only for investigating mechanisms of thrombus formation but also to assess the efficacy of anti-thrombotic drugs within the vasculature. | |||||
言語 | en | |||||
注記 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 浜松医科大学学位論文 医博第515号(平成20年3月17日) | |||||
言語 | ja | |||||
学位名 | ||||||
言語 | ja | |||||
学位名 | 博士(医学) | |||||
学位の区分 | ||||||
内容記述タイプ | Other | |||||
内容記述 | doctoral | |||||
言語 | en | |||||
学位の分野 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 医学系研究科 | |||||
言語 | ja | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 13802 | |||||
言語 | ja | |||||
学位授与機関名 | 浜松医科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2008-03-17 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第515号 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0031-6768 | |||||
EISSN | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 1432-2013 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 18553102 | |||||
出版社DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1007/s00424-008-0466-9 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |