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Frequent Detection of Y Chromosome Sequences in Japanese Turner's Syndrome by Southern Blot Analysis of Amplified DNA Nakagawa, Yuichi Natsume, Hiromune Kubota, Akira Takeuchi, Hiromi Nasuda, Kaoru Toya, Kazuhiko Endou, Akira Saegusa, Hirokazu Takeuchi, Rika Ogawa, Haruo Igarashi, Yoshio The Japanese Society for Pediatric Endocrinology 本文データは学協会の許諾に基づきJournal Archiveから複製したものである Turner syndrome, Y chromosome, PCR, Southern blot In Turner's syndrome, the detection of Y chromosome sequences is very important because the presence of the Y chromosome increases the risk of gonadoblastoma induction. Some recent studies have indicated the presence of the Y chromosome in patients with Turner's syndrome, by methods in which the Y chromosome was not detected by a standard cytogenetic method, but these studies concentrated on a part of the Y chromosome or used an insensitive methods. In this study, we approached the detection of Y chromosome sequences by a sensitive method, polymerase chain reaction (PCR) with seven pairs of primers that span the Y chromosome, followed by Southern blot analysis, in eleven Japanese patients with Turner's syndrome. Ten of the eleven patients had some Y chromosome sequences determined by PCR followed by Southern blot. This suggests that most patients with Turner's syndrome have cryptic Y chromosomes, but further studies and long-term follow-up of patients with the Y chromosome are needed to support our data and to clarify the significance of a small amount of Y chromosome. 日本小児内分泌学会 1996 eng journal article VoR http://hdl.handle.net/10271/2512 https://hama-med.repo.nii.ac.jp/records/2444 10.1297/cpe.5.83 09185739 13477358 Clinical Pediatric Endocrinology 5 2 83 87 https://hama-med.repo.nii.ac.jp/record/2444/files/Clinical P-5-83.pdf application/pdf 1.9 MB 2018-08-27