2026-03-08T01:16:49Z https://hama-med.repo.nii.ac.jp/oai

oai:hama-med.repo.nii.ac.jp:00003532 2023-10-17T05:05:23Z 2:15:17

Distinct spatial localization of three types of phosphatidyl choline in rat buccal mucosa identified by matrix-assisted laser desorption/ionization imaging mass spectrometry マトリックス支援レーザー脱離イオン化質量分析イメージングにより明らかになったラット頬粘膜における3種類のリン脂質の異なる分布 宮城, 摩里子 open access imaging mass spectrometry buccal mucosa phosphatidylcholine mass microscopy matrix assisted laser desorption/ionization doctoral 医学系研究科 The buccal mucosa is an inner lining exposed to frequent friction caused by teeth occlusion and therefore, a vulnerable site to lesions such as stomatitis, leukoplakia, lichen planus and cancer. Although it is considered an important tissue in the field of oral surgery, not enough information regarding the buccal mucosa is available. In this study, we characterized the buccal mucosa in the oral cavity using imaging mass spectrometry (IMS). Tissues such as epithelium, lamina propria and muscle were identified in rat buccal mucosa by HE staining. Moreover, IMS data of high-intensity ions were classified into 4 groups according to their distribution. In a previous IMS analysis of mouse tongue tissue, we detected linoleic acid-containing phosphatidyl choline (PC)(diacyl 16:0/18:2), oleic acid-containing PC(diacyl 16:0/18:1) and DHA-containing PC(diacyl 16:0/22:6) as major PCs. In the present work, we analyzed buccal mucosa tissue with emphasis on the aforementioned PC by IMS and showed that the investigated PCs existed in the layers of epithelium, lamina propria, and muscle at different ratios. This is the first study to analyze by IMS the buccal mucosa. The results presented here are likely to provide the perspective on understanding oral environments and to develop treatments for oral disorders. 日本医用マススペクトル学会 Japannese Society for Biomedical Mass Spectrometry 2017-06 eng doctoral thesis VoR http://hdl.handle.net/10271/00003669 https://hama-med.repo.nii.ac.jp/records/3532 https://doi.org/10.24508/mms.2017.06.002 2432-7441 2432-745X Medical Mass Spectrometry 1 1 2 9 甲第805号 博士（医学） 2019-03-13 13802 浜松医科大学 https://hama-med.repo.nii.ac.jp/record/3532/files/DT_805ronbun.pdf application/pdf 5.7 MB 2019-12-05