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SENSITIVE DETERMINATION OF ACETONITRILE AND PROPIONITRILE IN HUMAN WHOLE BLOOD BY HEADSPACE CAPILLARY GAS CHROMATOGRAPHY WITH CRYOGENIC OVEN TRAPPING 低温オーブントラッピングキャピラリーガスクロマトグラフィーによるヒト全血中アセトニトリル及びプロピオニトリルの高感度分析 Kaneko, Rina Fukui, Rena Watanabe, Kanako Hirata, Yukari Hamajima, Makoto Ishii, Akira Copyright 日本法中毒学会 本文データは日本法中毒学会の許諾のもと掲載しています。 Acetonitrile Propionitrile Gas chromatography Cryogenic oven trapping Flame thermionic detector (FTD) NPD We have developed a simple and sensitive method for determining acetonitrile and propionitrile in human whole blood using capillary gas chromatography (GC) with cryogenic oven trapping. After heating 0.5 ml of a whole blood sample containing both nitriles and 0.5 ml of distilled water in a 7-ml vial at 75℃ for 15 min, 5 ml of the headspace vapor was drawn into a 10-ml syringe and injected to GC with a flame thermionic detector. A sharp peak was obtained for acetonitrile at -10℃ of the initial oven temperature. The extraction efficiencies for acetonitrile and propionitrile were 0.27 and 0.40%, respectively. For quantitation of acetonitrile, propionitrile was used as internal standard, and vise versa. The calibration curves for both compounds gave good linearity in the range of 0.2-6 μg/ml whole blood. The detection limits (signal to noise ratio = 3) were 0.05μg/ml for acetonitrile and 0.01μg/ml for propionitrile. The coefficients of intra-day variations were 1.0% at 0.4μg/ml and 1.7% at 2μg/ml for acetonitrile; 7.1% at 0.4μg/m1 and 1.0 % at 2μg/ml for propionitrile. This method can be applicable for determining these nitriles in whole blood samples in acute and chronic intoxication cases. 日本法中毒学会 2005-01-31 eng journal article VoR http://hdl.handle.net/10271/1751 https://hama-med.repo.nii.ac.jp/records/1718 09159606 法中毒 Japanese journal of forensic toxicology 23 1 37 40 https://hama-med.repo.nii.ac.jp/record/1718/files/FT-23-37.pdf application/pdf 280.2 kB 2018-08-27