2026-03-13T05:23:55Z https://hama-med.repo.nii.ac.jp/oai

oai:hama-med.repo.nii.ac.jp:00002054 2023-08-02T07:29:16Z 1:11

Trans-Serosal Leakage of Proinflammatory Mediators during Abdominal Aortic Aneurysm Repair: Role of Phospholipase A2in Activating Leukocytes Sagara, Daisuke Unno, Naoki Yamamoto, Naoto Suzuki, Minoru Nishiyama, Motohiro Tanaka, Hiroki Konno, Hiroyuki Mitsuoka, Hiroshi Copyright 2010 Japanese College of Angiology, The Japanese Society for Vascular Surgery, Japanese Society of Phlebology 本文データは学協会の許諾に基づきJ-stageから複製したものである gut barrier function abdominal aortic aneurysm permeability peritoneum phospholipase A2 Gut barrier failure and the resultant translocation of luminal bacteria and bacterial products into the systemic circulation have been proposed as pathogenic mechanisms of multiorgan dysfunction syndrome (MODS) in open repair of abdominal aortic aneurysm (AAA). Our study aimed to demonstrate the direct release of gut-derived inflammatory mediators via the trans-serosal route in humans. Fifteen patients who underwent elective infrarenal open repair of AAA were randomized into two groups. In Group I patients (n = 10), the small intestine was exteriorized into a bowel bag. In Group II patients (n = 5), the small intestine was packed within the peritoneal cavity using large gauzes. We collected the bowel bag fluid in Group I and the ascites fluid, squeezed out from the gauzes at the end of surgery, in Group II. Leukocytes were collected from patients’ blood samples. Incubation with the bowel bag fluid and ascites fluid caused a significant increase in both granulocyte pseudopod formation and CD11b expression compared to that with control fluid (p < 0.01). The addition of phospholipase A2 (PLA2) inhibitor quinacrine abolished leukocyte activation by the bowel bag fluid. Based on these results, we consider that trasns-serosal leakage of gut-derived mediators occurred during the open repair of AAA; further, sPLA2 may be the most potent mediator in the activation of leukocytes among such gut-derived mediators in AAA surgery. Japanese College of Angiology, The Japanese Society for Vascular Surgery, Japanese Society of Phlebology 2010-08-24 eng journal article VoR http://hdl.handle.net/10271/2093 https://hama-med.repo.nii.ac.jp/records/2054 10.3400/avd.oa00930 1881641X 18816428 Annals of Vascular Diseases 3 2 131 139 https://hama-med.repo.nii.ac.jp/record/2054/files/AnnVascDis3_2-131.pdf application/pdf 287.0 kB 2018-08-27