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oai:hama-med.repo.nii.ac.jp:00002418 2023-08-02T05:01:55Z 1:11

Changes in the Subcellular Distribution of Free Carnitine and Its Acyl Derivatives in Diabetic Rat Hearts Following Treatment with L-Carnitine Aoshima, Shigeyuki Fujisawa, Shigeki Kobayashi, Akira Free carnitine Long-chain acylcarnitine Cellular distribution Mitochondria Diabetic heart L-carnitine treatment Carnitine deficiency has been demonstrated in diabetic hearts, and it is also well known that L-carnitine administration has a beneficial effect on cardiac function. Carnitine treatment would be expected to reduce the accumulation of long-chain acylcarnitine. However, many reports have shown that myocardial long-chain acylcarnitine levels were increased following treatment with L-carnitine in whole-heart studies. Since acylcarnitine exists in both the mitochondrial and cytosolic compartments, it is difficult to investigate changes in subcellular distribution by studying whole-heart preparations. The present study investigated the myocardial subcellular distribution of carnitine and its acyl derivatives in diabetic rats with or without L-carnitine treatment. Approximately 90% of total cellular carnitine was located in the cytosol in the diabetic hearts. Both mitochondrial and cytosolic levels of free carnitine and short-chain acylcarnitine were significantly decreased in the diabetic heart. However, the mitochondrial level of long-chain acylcarnitine was significantly increased. Lcarnitine treatment reduced the mitochondrial level of long-chain acylcarnitine, but the cytosolic level of long-chain acylcarnitine was significantly increased. These results show that L-carnitine treatment prevents the accumulation of long-chain acylcarnitine in the mitochondrial space and then reduces the detergent effect of long-chain acylcarnitine on the mitochondrial membrane. We suggest that it is a possible mechanism of the beneficial effect of L-carnitine treatment on the diabetic heart. (Jpn Heart J 34: 763-772, 1993) journal article International Heart Journal Association 1993-05-10 VoR application/pdf Japanese Heart Journal 6 34 763 772 00214868 1348673X https://hama-med.repo.nii.ac.jp/record/2418/files/JHeartJ-34-763.pdf http://hdl.handle.net/10271/2483 https://hama-med.repo.nii.ac.jp/records/2418 eng 10.1536/ihj.34.763 by International Heart Journal Association