@article{oai:hama-med.repo.nii.ac.jp:00001691,
 author = {小嶋, 高志 and 石井, 晃 and 勝又, 義直 and 鈴木, 加奈子 and 妹尾, 洋 and 鈴木, 修 and 熊澤, 武志},
 issue = {2},
 journal = {法中毒, Japanese journal of forensic toxicology},
 month = {May},
 note = {Halothane, enflurane, isoflurane and sevoflurane are widely used as general anaesthetics. Their toxic concentrations are only 2 - 4 times higher compared to their therapeutic concentrations, and their abusers are frequently reported. Thus sensitive determinations for these anaesthetics must be important. In this presentation, we have applied cryogenic oven trapping for determining above four general anaesthetics in human whole blood samples. A 0.5-ml human whole blood with or without anaesthetics was added to a 7-ml vial containing 0.48 ml of distilled water and 0.02 ml of 10 % Triton X-100 solution (containing 5 μg internal standard), and the mixture was heated at 55°C for 15 min. A 10-ml headspace vapor was introduced into a GC capillary column in the splitless mode at -40°C oven temperature, that was programmed up to 250°C for GC analysis with flame ionization detection. All four peaks of the anaesthetics were clearly separated, and the backgrounds were very clean. Their extraction efficiencies were 8.3 - 15.3 %. The calibration curves showed good linearity in the range of 0.5 - 20 μg/ml and the determination limits were 20 100 ng/ml, which were comparable to previous GC-mass spectrometric (MS) and GC-Fourier transform infrared spectroscopy (FTIR) methods. Our present method should be useful for forensic toxicology; it can be also rapid monitoring for their anaesthetics in the human blood.},
 pages = {134--135},
 title = {低温オーブントラッピングキャピラリーガスクロマトグラフィーによるヒト血中全身麻酔薬の高感度分析},
 volume = {18},
 year = {2000}
}