@article{oai:hama-med.repo.nii.ac.jp:00001703,
 author = {南方, かよ子 and 渡部, 加奈子 and 野澤, 秀樹 and 鈴木, 修 and 鈴木, 加奈子},
 issue = {2},
 journal = {法中毒, Japanese journal of forensic toxicology},
 month = {May},
 note = {A colorimetric method which is sensitive and specific to Mn has not been reported yet. A conventional flame atomic absorption method requires 1 g of liver containing 1ppm Mn, which is the highest concentration among soft tissues. Application of ESR method is examined in the present work because Mn(+2) is paramagnetic and can be measured by ESR method. Mn takes only +2 state in acid solution although it has several valence states from -2 to +7. In ESR spectrum Mn(+2) in acid solution shows characteristic 6 lines. The gvalue of the 3rd and 4th lines are 2.037 and 1.979, respectively, and the hyperfine splitting between them is 9.6 mT. Tissues were wet-ashed with conc nitric acid, and the diluted ashed solution was put into a quartz capillary and measured by an ESR spectrometer. In osteogenic disorder Shionogi rats intoxicated with paraquat, the Mn level was decreased to 0.6-fold of the control in liver and 0.7-fold in kidney, respectively. For the quantification, 1 mg of wet liver or kidney is enough,and the limit of detection is 60 pg of Mn. The ashed sample is stable for more than a year. Unlike the atomic absorption or emission method where the samples is burned out, the sample used in ESR method can be used for another purpose.},
 pages = {196--197},
 title = {電子スピン共鳴を用いたマンガンの微量定量法},
 volume = {20},
 year = {2002}
}