@article{oai:hama-med.repo.nii.ac.jp:00001709,
 author = {南方, かよ子 and 野澤, 秀樹 and 渡部, 加奈子 and 鈴木, 修 and 鈴木, 加奈子},
 issue = {2},
 journal = {法中毒, Japanese journal of forensic toxicology},
 month = {May},
 note = {A simple and sensitive method for the determination of nitrite has been developed. Nitrite can easily be reduced to NO radical by weak reductants such as ascorbic acid, I- and V3+ at room temperature. Some NO detection systems, already applied to the quantitation of nitrite, are chemiluminescence assay and ESR assay at 77 K after the trapping NO by hemoglobin. Our ESR method is based on the trapping NO by a metal-chelator complex consisting of ferrous ion(Fe2+) and diethyldithiocarbamic acid(DDC) in cycohexanol. The complex NO-Fe2+-(DDC)2 shows a characteristic three-line hyperfine structure(αN = 1.26 mT) with g value of 2.040 at room temperature. One sample can be quantitated within 5 min. When the sample tube was sealed from air with patty, the signal intensity remained constant for 30 days under room light at room temperature. By use of the present ESR method, NO at the concentration from 0.35 μM to 100 μM in 6 μｌ of cycohexanol is quantitated with the detection limit of 0.1 ng.},
 pages = {144--145},
 title = {電子スピン共鳴法を用いた亜硫酸イオンの定量法},
 volume = {21},
 year = {2003}
}