{"created":"2023-06-20T15:47:18.213172+00:00","id":1846,"links":{},"metadata":{"_buckets":{"deposit":"6ac7ea33-801d-400c-b13e-97a1128b478d"},"_deposit":{"created_by":4,"id":"1846","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"1846"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00001846","sets":["1:11"]},"author_link":["4577","4578","4579","4580","4581","4582"],"item_3_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"蛋白ホスファターゼ阻害因子-1は筋小胞体のCa+負荷をCa2+放出を変化させることなく蛋白キナーゼA依存性に増加させる","subitem_alternative_title_language":"ja"}]},"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"1140","bibliographicPageStart":"1133","bibliographicVolumeNumber":"73","bibliographic_titles":[{"bibliographic_title":"Circulation Journal","bibliographic_titleLang":"en"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: An increase in cytosolic protein phosphatases (PPs) de-phosphorylates phospholamban, decreasing the Ca2+ uptake of the sarcoplasmic reticulum (SR). The effects of PP inhibitors on cellular Ca2+ handling were investigated. Methods and Results: Twitch Ca2+ transients (CaTs) and cell shortening were measured in intact rat cardiac myocytes, and caffeine-induced Ca2+ transients (CaffCaTs) and Ca2+ sparks were studied in saponin-permeabilized cells. Calyculin A augmented isoproterenol-induced increases in CaTs and cell shortening without altering the diastolic [Ca2+]i and twitch [Ca2+]i decay. The protein kinase A catalytic subunit (PKAcat) increased the peak of CaffCaTs between 5 and 50 U/ml, and the addition of inhibitor-1 (I-1) augmented the increase. PKAcat increased Ca2+ spark frequency and the addition of I-1 increased it further. PKAcat at 50 U/ml amplified the peak and prolonged the duration of Ca2+ sparks, whereas the addition of I-1 did not alter them. An abrupt inhibition of SR Ca2+ uptake following exposure to PKAcat caused a gradual decrease in Ca2+ spark frequency, but the addition of I-1 did not accelerate the decline of Ca2+ spark frequency or CaffCaTs. Conclusions: Inhibition of PPs augmented the inotropic effect of isoproterenol. Specific inhibition of PP1 could stimulate the Ca2+ uptake of the SR with less significant effects on the Ca2+ release.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"THE JAPANESE CIRCULATION SOCIETY","subitem_publisher_language":"en"}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1253/circj.CJ-08-0871","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright 2009 THE JAPANESE CIRCULATION SOCIETY","subitem_rights_language":"en"},{"subitem_rights":"本文データは学協会の許諾に基づきJ-STAGEから複製したものである","subitem_rights_language":"ja"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13469843","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13474820","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawashima, Hirotaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satoh, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saotome, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Urushida, Tsuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Katoh, Hideki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayashi, Hideharu","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"CircJ-73-1133.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CircJ-73-1133.pdf","url":"https://hama-med.repo.nii.ac.jp/record/1846/files/CircJ-73-1133.pdf"},"version_id":"a6e38680-d90d-4350-95c6-64c282c2ead0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Calyculin A","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Inhibitor-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phospholamban","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein phosphatases","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sarcoplasmic reticulum","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Protein Phosphatase Inhibitor-1 Augments a Protein Kinase A-Dependent Increase in the Ca2+ Loading of the Sarcoplasmic Reticulum Without Changing Its Ca2+ Release","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Protein Phosphatase Inhibitor-1 Augments a Protein Kinase A-Dependent Increase in the Ca2+ Loading of the Sarcoplasmic Reticulum Without Changing Its Ca2+ Release","subitem_title_language":"en"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"1846","relation_version_is_last":true,"title":["Protein Phosphatase Inhibitor-1 Augments a Protein Kinase A-Dependent Increase in the Ca2+ Loading of the Sarcoplasmic Reticulum Without Changing Its Ca2+ Release"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-02T01:30:49.839444+00:00"}