{"created":"2023-06-20T15:47:24.652508+00:00","id":1947,"links":{},"metadata":{"_buckets":{"deposit":"afbe7788-2ba7-4c2a-8c6e-6dce9b6c423e"},"_deposit":{"created_by":4,"id":"1947","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"1947"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00001947","sets":["1:11"]},"author_link":["4939"],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1987-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"546","bibliographicPageStart":"539","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"日本産科婦人科學會雜誌","bibliographic_titleLang":"ja"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"甲状腺機能冗進症を呈した絨毛性疾患患者尿よりIR-hCGを抽出・精製し,invitro甲状腺スライス実験を行ない hyrotropic activityを有する画分の生化学的性質につき検討した.1.絨毛性疾患患者14症例のうちhyperthyroidismを呈した3症例からthyrotropic activityを有する画分(GB-D_2-S-fraction(3))を抽出6精製することができた、2.GBD_2-S-fraction(3)(hCT)の分子量は35,000でhCGと同様にαとβ画分に分離可能であり,hCT-αは15,000,hCT-βは20,000の分子量を有した.3.甲状腺スライス実験の結果,hCT添加によりmedium中cAMP,組織中cAMPおよびmedium中T_3,T_4の増加を認め,thyrotropic activityを確認した.4. 再構成実験によるthyrotropic avtivityの再現性は,hCT-β subunitとhCT-α subunitを再構成した場合のみthyrotropic activityを認めた.しかし,hCT-β subunit単独では活性を認めなかった.5. hCTおよびhCGのcarboxypeptidaseによるdigestion実験の結果,hCGにCPBおよびCPYを反応させると,Ile, Leu, Ser, Glu, Pro, Arg, Lysが優位に分解されるが,hCTに対してはcarboxypeptidaseが作用しなかった.以上の結果より,hCTは免疫学的にhCGに非常に近いものであるが,hCT-β subunitのC末端のうち,少なくともcarboxypeptidaseが作用するはずのIle-Leu-Pro-Glu残基を含めた一部のcomponentが欠如しているか,分子のcomformational changeを起こして,throtropic activityを発揮するようになったものと考えられる","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本産科婦人科学会","subitem_publisher_language":"ja"}]},"item_3_relation_22":{"attribute_name":"NII論文ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110002119560","subitem_relation_type_select":"NAID"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright 日本産科婦人科学会","subitem_rights_language":"ja"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである","subitem_rights_language":"ja"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03009165","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 浩","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"4939","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"sankafujinka-39_4-539.pdf","filesize":[{"value":"775.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"sankafujinka-39_4-539.pdf","url":"https://hama-med.repo.nii.ac.jp/record/1947/files/sankafujinka-39_4-539.pdf"},"version_id":"b731542e-9d06-4054-a312-2d4f95cb827c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Carboxypeptidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gonadotropin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Thyrotropic activity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Trophoblastic disease","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"絨毛性疾患患者尿中のimmunoreactive-human Chorionic Gonadotropin におけるthyrotropic activityに関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"絨毛性疾患患者尿中のimmunoreactive-human Chorionic Gonadotropin におけるthyrotropic activityに関する研究","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"1947","relation_version_is_last":true,"title":["絨毛性疾患患者尿中のimmunoreactive-human Chorionic Gonadotropin におけるthyrotropic activityに関する研究"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-02T07:20:26.963318+00:00"}