{"created":"2024-09-04T01:59:06.817477+00:00","id":2000200,"links":{},"metadata":{"_buckets":{"deposit":"cab321be-012d-4e3f-b06b-bc7dcf335920"},"_deposit":{"created_by":4,"id":"2000200","owner":"4","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"2000200"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:02000200","sets":["2:15:17"]},"author_link":[],"control_number":"2000200","item_5_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"UBL3は細胞内でα-シヌクレインと相互作用し、その相互作用はEGFR経路阻害剤オシメルチニブによってダウンレギュレーションされる","subitem_alternative_title_language":"ja"}]},"item_5_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicNumberOfPages":"16","bibliographicPageStart":"1685","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Biomedicines","bibliographic_titleLang":"en"}]}]},"item_5_date_granted_17":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-09-22"}]},"item_5_degree_grantor_15":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"浜松医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13802","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_12":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_5_description_13":{"attribute_name":"学位の区分","attribute_value_mlt":[{"subitem_description":"doctoral","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_description_14":{"attribute_name":"学位の分野","attribute_value_mlt":[{"subitem_description":"医学系研究科","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ubiquitin-like 3 (UBL3) acts as a post-translational modification (PTM) factor and regulates protein sorting into small extracellular vesicles (sEVs). sEVs have been reported as vectors for the pathology propagation of neurodegenerative diseases, such as α-synucleinopathies. Alpha-synuclein (α-syn) has been widely studied for its involvement in α-synucleinopathies. However, it is still unknown whether UBL3 interacts with α-syn, and is influenced by drugs or compounds. In this study, we investigated the interaction between UBL3 and α-syn, and any ensuing possible functional and pathological implications. We found that UBL3 can interact with α-syn by the Gaussia princeps based split luciferase complementation assay in cells and immunoprecipitation, while cysteine residues at its C-terminal, which are considered important as PTM factors for UBL3, were not essential for the interaction. The interaction was upregulated by 1-methyl-4-phenylpyridinium exposure. In drug screen results, the interaction was significantly downregulated by the treatment of osimertinib. These results suggest that UBL3 interacts with α-syn in cells and is significantly downregulated by epidermal growth factor receptor (EGFR) pathway inhibitor osimertinib. Therefore, the UBL3 pathway may be a new therapeutic target for α-synucleinopathies in the future.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_dissertation_number_18":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第952号"}]},"item_5_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_5_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"37371780","subitem_relation_type_select":"PMID"}}]},"item_5_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/biomedicines11061685","subitem_relation_type_select":"DOI"}}]},"item_5_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_5_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"2227-9059","subitem_source_identifier_type":"EISSN"}]},"item_5_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chen, Bin","creatorNameLang":"en"},{"creatorName":"チェン, ビン","creatorNameLang":"ja-Kana"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-09-04"}],"displaytype":"detail","filename":"DT_952ronbun.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"label":"論文本文","objectType":"fulltext","url":"https://hama-med.repo.nii.ac.jp/record/2000200/files/DT_952ronbun.pdf"},"version_id":"6ad7000b-4e4b-4e90-911b-5f8af9e55c04"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"UBL3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"α-synuclein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"interaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"drug screen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"EGFR pathway inhibitor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osimertinib","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"downregulate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"α-synucleinopathies","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"UBL3 interacts with alpha-synuclein in cells and the interaction is downregulated by the EGFR pathway inhibitor osimertinib","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"UBL3 interacts with alpha-synuclein in cells and the interaction is downregulated by the EGFR pathway inhibitor osimertinib","subitem_title_language":"en"}]},"item_type_id":"5","owner":"4","path":["17"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-09-04"},"publish_date":"2024-09-04","publish_status":"0","recid":"2000200","relation_version_is_last":true,"title":["UBL3 interacts with alpha-synuclein in cells and the interaction is downregulated by the EGFR pathway inhibitor osimertinib"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2024-10-04T01:51:32.941407+00:00"}