{"created":"2025-01-14T01:38:52.178427+00:00","id":2000317,"links":{},"metadata":{"_buckets":{"deposit":"7ccb5001-9826-4d44-b553-6c3c5fc16b56"},"_deposit":{"created_by":4,"id":"2000317","owner":"4","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"2000317"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:02000317","sets":["2:15:17"]},"author_link":[],"control_number":"2000317","item_5_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ヒト肺腺がんにおいて腫瘍抑制遺伝子であるFHITの発現はLINC00173-SNAIL軸によって制御される","subitem_alternative_title_language":"ja"}]},"item_5_biblio_info_5":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-11-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"23","bibliographicPageStart":"17011","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences","bibliographic_titleLang":"en"}]}]},"item_5_date_granted_17":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-03-13"}]},"item_5_degree_grantor_15":{"attribute_name":"item_5_degree_grantor_15","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"浜松医科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Hamamatsu University School of Medicine"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13802","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_12":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_5_description_13":{"attribute_name":"item_5_description_13","attribute_value_mlt":[{"subitem_description":"doctoral","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_description_14":{"attribute_name":"item_5_description_14","attribute_value_mlt":[{"subitem_description":"医学系研究科","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Long non-coding RNAs (lncRNAs) play a critical role in a variety of human diseases such as cancer. Here, to elucidate a novel function of a lncRNA called LINC00173, we investigated its binding partner, target gene, and its regulatory mechanism in lung adenocarcinoma, including the A549 cell line and patients. In the A549 cell line, RNA immunoprecipitation (RIP) assays revealed that LINC00173 efficiently binds to SNAIL. RNA-seq and RT-qPCR analyses revealed that the expression of FHIT was decreased upon LINC00173 depletion, indicating that FHIT is a target gene of LINC00173. Overexpression of SNAIL suppressed and depletion of SNAIL increased the expression of FHIT, indicating that SNAIL negatively regulates FHIT. The downregulation of FHIT expression upon LINC00173 depletion was restored by additional SNAIL depletion, revealing a LINC00173-SNAILFHIT axis for FHIT regulation. Data from 501 patients with lung adenocarcinoma also support the existence of a LINC00173-SNAIL-FHIT axis, as FHIT expression correlated positively with LINC00173 (p=1.75×10 -6) and negatively with SNAIL (p=7.00×10 -5). Taken together, we propose that LINC00173 positively regulates FHIT gene expression by binding to SNAIL and inhibiting its function in human lung adenocarcinoma. Thus, this study sheds light on the LINC00173-SNAIL-FHIT axis, which may be a key mechanism for carcinogenesis and progression in human lung adenocarcinoma.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_dissertation_number_18":{"attribute_name":"dissertation_number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第969号"}]},"item_5_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI","subitem_publisher_language":"en"}]},"item_5_relation_26":{"attribute_name":"item_5_relation_26","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"38069335","subitem_relation_type_select":"PMID"}}]},"item_5_relation_28":{"attribute_name":"item_5_relation_28","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/ijms242317011","subitem_relation_type_select":"DOI"}}]},"item_5_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(C) 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).","subitem_rights_language":"en"}]},"item_5_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"EISSN"}]},"item_5_source_id_23":{"attribute_name":"item_5_source_id_23","attribute_value_mlt":[{"subitem_source_identifier":"AA12038549","subitem_source_identifier_type":"NCID"}]},"item_5_version_type_32":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 貴人","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"スズキ, タカヒト","creatorNameLang":"ja-Kana","creatorNameType":"Personal"},{"creatorName":"Suzuki, Takahito","creatorNameLang":"en","creatorNameType":"Personal"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-01-14"}],"displaytype":"detail","filename":"DT_969ronbun.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"論文本文","objectType":"fulltext","url":"https://hama-med.repo.nii.ac.jp/record/2000317/files/DT_969ronbun.pdf"},"version_id":"39b43ad8-b452-43e1-9e05-6019fc20b576"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"lncRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"LINC00173","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SNAIL","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FHIT","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lung adenocarcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tumor suppressor gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Expression of tumor suppressor FHIT is regulated by the LINC00173-SNAIL axis in human lung adenocarcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of tumor suppressor FHIT is regulated by the LINC00173-SNAIL axis in human lung adenocarcinoma","subitem_title_language":"en"}]},"item_type_id":"5","owner":"4","path":["17"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-02-10"},"publish_date":"2025-02-10","publish_status":"0","recid":"2000317","relation_version_is_last":true,"title":["Expression of tumor suppressor FHIT is regulated by the LINC00173-SNAIL axis in human lung adenocarcinoma"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2025-02-26T02:22:31.316645+00:00"}