{"created":"2025-06-25T02:25:13.329877+00:00","id":2000461,"links":{},"metadata":{"_buckets":{"deposit":"9874e1f1-ae2b-426a-8ed8-8bb482a0571a"},"_deposit":{"created_by":4,"id":"2000461","owner":"4","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"2000461"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:02000461","sets":["2:15:17"]},"author_link":[],"control_number":"2000461","item_1747115756369":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"38899899","subitem_relation_type_select":"PMID"}}]},"item_1747115938917":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1128/jvi.00522-24","subitem_relation_type_select":"DOI"}}]},"item_1747116992798":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00708779","subitem_source_identifier_type":"NCID"}]},"item_1747119125853":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"IMPORTANCE\nAlthough basic research on the HCV life cycle has progressed significantly over the past two decades, our understanding of the molecular mechanisms that regulate the process of particle formation, in particular encapsidation of the genome or nucleocapsid assembly, has been limited. We present here, for the first time, that two RNA-binding proteins, RPL17 and YBX1, bind to the 3′X in the 3′UTR of the HCV genome, which potentially acts as a packaging signal, and facilitates the viral particle assembly. Our study revealed that RPL17 and YBX1 exert a positive effect on the interaction between HCV RNA and Core protein, suggesting that the presence of both host factors modulate an RNA structure or conformation suitable for packaging the viral genome. These findings help us to elucidate not only the regulatory mechanism of the particle assembly of HCV but also the function of host RNA-binding proteins during viral infection.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1747119187110":{"attribute_name":"学位の区分","attribute_value_mlt":[{"subitem_description":"doctoral","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_1747119200876":{"attribute_name":"学位の分野","attribute_value_mlt":[{"subitem_description":"医学系研究科","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_1748498410707":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_1749433886912":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1098-5514","subitem_source_identifier_type":"EISSN"}]},"item_1750750575061":{"attribute_name":"著者情報","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"リウ, ジエ","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"刘, 杰","creatorNameLang":"zh-tw","creatorNameType":"Personal"}]}]},"item_1751527345221":{"attribute_name":"学位論文要旨/本文へのリンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"学位論文要旨"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10271/0002000241","subitem_relation_type_select":"HDL"}}]},"item_30001_access_rights4":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_30001_alternative_title1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"リボソームタンパク質L17とYボックス結合タンパク質1はC型肝炎ウイルスの3'非翻訳領域との相互作用を介してウイルスのアセンブリに関与する","subitem_alternative_title_language":"ja"}]},"item_30001_bibliographic_information17":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-06-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageStart":"e00522-24","bibliographicVolumeNumber":"98","bibliographic_titles":[{"bibliographic_title":"Journal of Virology","bibliographic_titleLang":"en"}]}]},"item_30001_creator2":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000089376696","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000089376696"}],"affiliationNames":[{"affiliationName":"浜松医科大学","affiliationNameLang":"ja"},{"affiliationName":"Hamamatsu University School of Medicine","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Liu, Jie","creatorNameLang":"en","creatorNameType":"Personal"}]}]},"item_30001_date_granted20":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2024-07-19"}]},"item_30001_degree_grantor21":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"浜松医科大学"},{"subitem_degreegrantor_language":"en","subitem_degreegrantor_name":"Hamamatsu University School of Medicine"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13802","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_30001_degree_name19":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_30001_description8":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The 3′ untranslated region (3′UTR) of the hepatitis C virus (HCV) RNA genome, which contains a highly conserved 3′ region named the 3′X-tail, plays an essential role in RNA replication and promotes viral IRES-dependent translation. Although our previous work has found a cis-acting element for genome encapsidation within 3′X, there is limited information on the involvement of the 3′UTR in particle formation. In this study, proteomic analyses identified host cell proteins that bind to the 3′UTR containing the 3′X region but not to the sequence lacking the 3′X. Further characterization showed that RNA-binding proteins, ribosomal protein L17 (RPL17), and Y-box binding protein 1 (YBX1) facilitate the efficient production of infectious HCV particles in the virus infection cells. Using small interfering RNA (siRNA)-mediated gene silencing in four assays that distinguish between the various stages of the HCV life cycle, RPL17 and YBX1 were found to be most important for particle assembly in the trans-packaging assay with replication-defective subgenomic RNA. In vitro assays showed that RPL17 and YBX1 bind to the 3′UTR RNA and deletion of the 3′X region attenuates their interaction. Knockdown of RPL17 or YBX1 resulted in reducing the amount of HCV RNA co-precipitating with the viral Core protein by RNA immunoprecipitation and increasing the relative distance in space between Core and double-stranded RNA by confocal imaging, suggesting that RPL17 and YBX1 potentially affect HCV RNA-Core interaction, leading to efficient nucleocapsid assembly. These host factors provide new clues to understanding the molecular mechanisms that regulate HCV particle formation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_30001_dissertation_number18":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第989号"}]},"item_30001_file22":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-07-03"}],"displaytype":"detail","filename":"DT_989ronbun.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"label":"論文本文","objectType":"fulltext","url":"https://hama-med.repo.nii.ac.jp/record/2000461/files/DT_989ronbun.pdf"},"version_id":"5f1ba6c4-f361-456b-89da-48d62c710022"}]},"item_30001_language10":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30001_publisher9":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology","subitem_publisher_language":"en"}]},"item_30001_rights5":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright 2024 American Society for  Microbiology.","subitem_rights_language":"en"}]},"item_30001_source_identifier16":{"attribute_name":"PISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-538X","subitem_source_identifier_type":"PISSN"}]},"item_30001_subject7":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"hepatitis C virus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"particle assembly","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"encapsidation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"genome packaging","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RPL17","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"YBX1","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30001_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Involvement of ribosomal protein L17 and Y-Box binding protein 1 in assembly of hepatitis C virus potentially via their interaction with the 3’ untranslated region of the viral genome","subitem_title_language":"en"}]},"item_30001_version_type12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"Involvement of ribosomal protein L17 and Y-Box binding protein 1 in assembly of hepatitis C virus potentially via their interaction with the 3’ untranslated region of the viral genome","item_type_id":"40002","owner":"4","path":["17"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-07-18"},"publish_date":"2025-07-18","publish_status":"0","recid":"2000461","relation_version_is_last":true,"title":["Involvement of ribosomal protein L17 and Y-Box binding protein 1 in assembly of hepatitis C virus potentially via their interaction with the 3’ untranslated region of the viral genome"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2025-07-24T00:17:53.533539+00:00"}