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UBL3 overexpression enhances EV-mediated Achilles protein secretion in conditioned media of MDA-MB-231 cells
http://hdl.handle.net/10271/0002000467
http://hdl.handle.net/10271/00020004672bc38862-3f3c-44f9-b6a1-b1bdd57bf1a3
| 名前 / ファイル | ライセンス | アクション |
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論文本文 (6.6 MB)
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| Item type | 共通アイテムタイプ / Common item types(1) | |||||||||||
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| 公開日 | 2025-08-21 | |||||||||||
| タイトル | ||||||||||||
| タイトル | UBL3 overexpression enhances EV-mediated Achilles protein secretion in conditioned media of MDA-MB-231 cells | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題 | Extracellular vesicles | |||||||||||
| キーワード | ||||||||||||
| 主題 | UBL3 | |||||||||||
| キーワード | ||||||||||||
| 主題 | Ubiquitin | |||||||||||
| キーワード | ||||||||||||
| 主題 | Dual-reporter system | |||||||||||
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| 主題 | Akaluc | |||||||||||
| キーワード | ||||||||||||
| 主題 | Achilles | |||||||||||
| キーワード | ||||||||||||
| 主題 | Breast cancer | |||||||||||
| その他のタイトル | ||||||||||||
| その他のタイトル | UBL3の過剰発現は、MDA-MB-231細胞の条件培地におけるEVを介したアキレスタンパク質の分泌を促進する | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 著者 |
Mst. Afsana, Mimi
× Mst. Afsana, Mimi
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| 著者情報 |
エムエスティー. アフサナ, ミミ
× エムエスティー. アフサナ, ミミ
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| 書誌情報 |
en : Biochemical and Biophysical Research Communications 巻 738, p. 150559, 発行日 2024-12 |
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| 出版者 | ||||||||||||
| 出版者 | Elsevier | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | Copyright 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies. | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Cancer cells communicate within the tumor microenvironment (TME) through extracellular vesicles (EVs), which act as crucial messengers in intercellular communication, transporting biomolecules to facilitate cancer pro-gression. Ubiquitin-like 3 (UBL3) facilitates protein sorting into small EVs as a post-translational modifier. However, the effect of UBL3 overexpression in EV-mediated protein secretion has not been investigated yet. This study aimed to investigate the effect of UBL3 overexpression in enhancing EV-mediated Achilles protein secre-tion in MDA-MB-231 (MM) cells by a dual-reporter system integrating Akaluc and Achilles tagged with Ubiquitin where self-cleaving P2A linker connects Akaluc and Achilles. MM cells stably expressing Ubiquitin-Akaluc-P2A-Achilles (Ubi-Aka/Achi) were generated. In our study, both the bioluminescence of Ubiquitin-Akaluc (Ubi-Aka) and the fluorescence of Achilles secretion were observed. The intensity of Ubi-Aka was thirty times lower, while the Achilles was four times lower than the intensity of corresponding cells. The ratio of Ubi-Aka and Achilles in conditioned media (CM) was 7.5. They were also detected within EVs using an EV uptake luciferase assay and fluorescence imaging. To investigate the effect of the UBL3 overexpression in CM, Ubi-Aka/Achi was transiently transfected into MM-UBL3-KO, MM, and MM-Flag-UBL3 cells. We found that the relative fluorescence expression of Achilles in CM of MM-UBL3-KO, MM, and MM-Flag-UBL3 cells was 30%, 28%, and 45%, respectively. These findings demonstrated that UBL3 overexpression enhances EV-mediated Achilles protein secretion in CM of MM cells. Targeting UBL3 could lead to novel therapies for cancer metastasis by reducing the secretion of pro-metastatic proteins, thereby inhibiting disease progression. | |||||||||||
| 学位名 | ||||||||||||
| 学位名 | 博士（医学） | |||||||||||
| 学位の区分 | ||||||||||||
| 内容記述 | doctoral | |||||||||||
| 学位の分野 | ||||||||||||
| 内容記述 | 医学系研究科 | |||||||||||
| 学位授与機関 | ||||||||||||
| 学位授与機関名 | 浜松医科大学 | |||||||||||
| 学位授与機関名 | Hamamatsu University School of Medicine | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2024-09-20 | |||||||||||
| 学位授与番号 | ||||||||||||
| 学位授与番号 | 甲第997号 | |||||||||||
| PISSN | ||||||||||||
| 収録物識別子 | 0006-291X | |||||||||||
| EISSN | ||||||||||||
| 収録物識別子 | 1090-2104 | |||||||||||
| NII書誌ID (NCID) | ||||||||||||
| 収録物識別子 | AA11542044 | |||||||||||
| PubMed番号 (PMID) | ||||||||||||
| 関連識別子 | 39182355 | |||||||||||
| 出版社DOI | ||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.bbrc.2024.150559 | |||||||||||
| 学位論文要旨/本文へのリンク | ||||||||||||
| 関連識別子 | http://hdl.handle.net/10271/0002000250 | |||||||||||
| 関連名称 | 学位論文要旨 | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
