{"created":"2023-06-20T15:47:28.613240+00:00","id":2018,"links":{},"metadata":{"_buckets":{"deposit":"285c9c29-cfbb-4e5a-929e-9664f4073f47"},"_deposit":{"created_by":2,"id":"2018","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"2018"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00002018","sets":["1:11"]},"author_link":["5255","5257","5256","5254"],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-10-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"400","bibliographicPageStart":"397","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"Experimental Animals"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have established a genetic quality testing system for early stage embryos of the mouse. A method of preparation of template DNA for PCR was established using the lysis buffer (1 × PCR reaction buffer supplemented with proteinase K at a concentration of 40 μg/ml) developed by the authors. We demonstrated that two 8-cell embryos of an inbred strain provide sufficient volumes of template DNA for PCR to identify the strain of embryos using four microsatellite markers (D3Mit54, D5Mit18, D6Mit15 and D8Mit50) differentiating 13 inbred strains of mice. This system will be useful in embryo banks that have recently been established worldwide for demonstrating the genetic accuracy of a given strain prior to recovery of live animals.","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本実験動物学会"}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1538/expanim.52.397","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2003 Japanese Association for Laboratory Animal Science"},{"subitem_rights":"本文データは学協会の許諾に基づきJ-STAGEから複製したものである"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13411357","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18817122","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Katoh, Hideki"}],"nameIdentifiers":[{"nameIdentifier":"5254","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oda, Koji"}],"nameIdentifiers":[{"nameIdentifier":"5255","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hioki, Kyoji"}],"nameIdentifiers":[{"nameIdentifier":"5256","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Muguruma, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"5257","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"ExpAnim-52_5-397.pdf","filesize":[{"value":"27.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ExpAnim-52_5-397.pdf","url":"https://hama-med.repo.nii.ac.jp/record/2018/files/ExpAnim-52_5-397.pdf"},"version_id":"ea488ec0-3d0c-44a8-afd0-fa847a7fd408"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"early embryos","subitem_subject_scheme":"Other"},{"subitem_subject":"embryo bank","subitem_subject_scheme":"Other"},{"subitem_subject":"genetic quality","subitem_subject_scheme":"Other"},{"subitem_subject":"microsatellite markers","subitem_subject_scheme":"Other"},{"subitem_subject":"mouse","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Genetic Quality Testing System for Early Stage Embryos in the Mouse","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Genetic Quality Testing System for Early Stage Embryos in the Mouse"}]},"item_type_id":"3","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"2018","relation_version_is_last":true,"title":["A Genetic Quality Testing System for Early Stage Embryos in the Mouse"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-06-20T17:23:56.692566+00:00"}