{"created":"2023-06-20T15:47:28.819785+00:00","id":2021,"links":{},"metadata":{"_buckets":{"deposit":"3a867074-a491-435a-915e-a060ad6fd196"},"_deposit":{"created_by":2,"id":"2021","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"2021"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00002021","sets":["1:11"]},"author_link":["5267","5269","5266","5268","5270"],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-08-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"376","bibliographicPageStart":"373","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"Experimental Animals"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We attempted to determine the number of sperm cells required for genotyping of one microsatellite marker. The crude genomic DNA extracted from about 760 or more sperm cells gave sufficient quantity of PCR product using a 20 μl-scale PCR. We also studied the effects of non-ionic detergents on extraction of crude sperm genomic DNA. PCR products amplified with the crude sperm genomic DNA extracted using the lysis buffer supplemented with non-ionic detergents showed much clear bands. In conclusion, our results suggest that a small part of the frozen sperm, which is less than 1/10 of the original volume (10 μl), provides sufficient quantity of template DNA for genetic quality testing.","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本実験動物学会"}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1538/expanim.54.373","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2005 Japanese Association for Laboratory Animal Science"},{"subitem_rights":"本文データは学協会の許諾に基づきJ-STAGEから複製したものである"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13411357","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18817122","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Katoh, Hideki"}],"nameIdentifiers":[{"nameIdentifier":"5266","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshino, Sayaka"}],"nameIdentifiers":[{"nameIdentifier":"5267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inui, Yumiko"}],"nameIdentifiers":[{"nameIdentifier":"5268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Honda, Sayaka"}],"nameIdentifiers":[{"nameIdentifier":"5269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takabayashi, Shuji"}],"nameIdentifiers":[{"nameIdentifier":"5270","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"ExpAnim-54_4-373.pdf","filesize":[{"value":"105.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ExpAnim-54_4-373.pdf","url":"https://hama-med.repo.nii.ac.jp/record/2021/files/ExpAnim-54_4-373.pdf"},"version_id":"5fe21bc8-743d-449a-85d3-be15a075bc53"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"genetic quality testing","subitem_subject_scheme":"Other"},{"subitem_subject":"non-ionic detergents","subitem_subject_scheme":"Other"},{"subitem_subject":"sperm genomic DNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Microsatellite Genotyping for Genetic Quality Testing Using Sperm Cells in the Mouse","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Microsatellite Genotyping for Genetic Quality Testing Using Sperm Cells in the Mouse"}]},"item_type_id":"3","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"2021","relation_version_is_last":true,"title":["Microsatellite Genotyping for Genetic Quality Testing Using Sperm Cells in the Mouse"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-06-20T17:23:50.025640+00:00"}