{"created":"2023-06-20T15:47:41.084633+00:00","id":2220,"links":{},"metadata":{"_buckets":{"deposit":"990de027-7124-44a1-9ce7-756806cd3a95"},"_deposit":{"created_by":4,"id":"2220","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"2220"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00002220","sets":["1:11"]},"author_link":[],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-09-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1015","bibliographicPageStart":"1007","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"Japanese circulation journal"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This study was undertaken to examine the effects of hydrogen peroxide on stimulatory guanine nucleotide-binding protein (Gs), and coupling in the β-aderenergic receptor - Gs - adenylate cyclase system in rat heart, in vitro. Cardiac membranes were preincubated with various concentrations (0.1, 1 , and 10 mM) of hydrogen peroxide at 30℃ for 5, 10, 30 and 60 min. Although the assay of β-adrenergic receptors involving [^3H]-dihydroaloprenolol ([^3H]-DHA) binding revealed that the maximal number of binding sites (B_<max>) was not altered, the dissociation constant (K_d) for [^3H]-DHA was increased in the presence of I mM and 10 mM hydrogen peroxide (control 0.68±0.16 nM, vs 1 mM H202 1.13±0.16, 10 mM H202 1.01±0. 12). Conversely, no significant changes in Gs activities were observed in hydrogen peroxide-treated groups. Adenylate cyclase activity (stimulated by forskolin) was significantly reduced by 10 mM hydrogen peroxide after a 5 min preincubation period (control 277.1±19.2 pmol cAMP/mg protein/min, H202 230.3±14.9). The amounts of cyclic AMP produced by the stimulation of membranes with GTP, GTP + (1)-isoproterenol, guanylimidodiphosphate (Gpp(NH)p) or Gpp(NH)p+(1)-isoproterenol were significantly lower in 10 mM hydrogen peroxide-treated groups than those in controls (GTP: control 57.6±5.6pmol cAMP/mg protein/min vs H_2O_2 46.4±6.9, GTP+(1)-isoproterenol: control 83.9±10.2 vs H_2O_2 67.7±10.3, Gpp(NH)p: control 77.5±8.8 vs H_2O_2 61.0±8.6. Gpp(NH)p+(1)-isoproterenol: control 105.0±13.1 vs H202 83.9±12.2, forskolin: control 223.2±13.8 vs H202 182.8±18.4). In the presence of hydrogen peroxide, the extents of the depression in cAMP production induced by GTP. GTP+isoproterenol, Gpp(NH)p, and Gpp(NH)p+ (1)-isoproterenol were similar to those induced by forskolin stimulation. These results indicate that hydrogen peroxide does not affect Gs activity or coupling in the β-receptor - Gs - adenylate cyclase system, but does depress cAMP production by inhibiting adenylate cyclase.","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本循環器学会"}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1253/jcj.57.1007","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright 日本循環器学会"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00471828","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"増田, 尚道"}]},{"creatorNames":[{"creatorName":"Kaneko, Masanori"}]},{"creatorNames":[{"creatorName":"Rong, Bang Hong"}]},{"creatorNames":[{"creatorName":"Ikegaya, Takayoshi"}]},{"creatorNames":[{"creatorName":"Hayashi, Hideharu"}]},{"creatorNames":[{"creatorName":"Koobayashi, Akira"}]},{"creatorNames":[{"creatorName":"Yamazaki, Noboru"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"CircJ57_10-1007.pdf","filesize":[{"value":"872.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CircJ57_10-1007.pdf","url":"https://hama-med.repo.nii.ac.jp/record/2220/files/CircJ57_10-1007.pdf"},"version_id":"3c262ca4-7e69-489f-ad24-1c352d149808"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"β-adrenergic receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"Guanine nucleotide-binding protein","subitem_subject_scheme":"Other"},{"subitem_subject":"Adenylate cyclase","subitem_subject_scheme":"Other"},{"subitem_subject":"Oxygen free radicals","subitem_subject_scheme":"Other"},{"subitem_subject":"Hydrogen peroxide","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"EFFECTS OF HYDROGEN PEROXIDE ON STIMULATORY GUANINE NUCLEOTIDE-BINDING PROTEIN IN RAT HEART","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"EFFECTS OF HYDROGEN PEROXIDE ON STIMULATORY GUANINE NUCLEOTIDE-BINDING PROTEIN IN RAT HEART","subitem_title_language":"en"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"2220","relation_version_is_last":true,"title":["EFFECTS OF HYDROGEN PEROXIDE ON STIMULATORY GUANINE NUCLEOTIDE-BINDING PROTEIN IN RAT HEART"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-02T04:42:29.812359+00:00"}