{"created":"2023-06-20T15:47:51.206659+00:00","id":2381,"links":{},"metadata":{"_buckets":{"deposit":"1574ce62-16f5-429a-a889-876cc3b1b1c2"},"_deposit":{"created_by":4,"id":"2381","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"2381"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00002381","sets":["1:11"]},"author_link":[],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-04-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"101","bibliographicPageStart":"95","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"生物物理化学"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We investigated the effects of electrophoretic gels, buffers or temperature on SSCP pattern by an automated electrophoretic system, Phast System. The electrophoresis on the 12.5% homogeneous gel gave the greatest difference in the mobility shift by genetic mutation. The SDS-containing buffer increased the resolution of each band as well as the sensitivity of detection in most cases. However, for certain mutated sequences, the native buffer was superior at detecting mutations. The mobility shift is also temperature dependent irrespective of native or SDS buffer condition. In any new mutation sequences, it is difficult to determine which is the better condition to detect the mutations, SDS or native buffer; however, the SDS-containing buffer may lead to a better sensitivity of detection. In conclusion, it is important to perform SSCP analysis under SDS buffer condition as well as native buffer condition in all cases.","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本電気泳動学会"}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2198/sbk.38.95","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本電気泳動学会"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00319082","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13499785","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"前川, 真人"}]},{"creatorNames":[{"creatorName":"須藤, 加代子"}]},{"creatorNames":[{"creatorName":"Dilip, Chandra  Dey"}]},{"creatorNames":[{"creatorName":"小谷, 一夫"}]},{"creatorNames":[{"creatorName":"菅野, 剛史"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"Seibutubuturi38_2-95.pdf","filesize":[{"value":"4.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Seibutubuturi38_2-95.pdf","url":"https://hama-med.repo.nii.ac.jp/record/2381/files/Seibutubuturi38_2-95.pdf"},"version_id":"0bfa5e73-9e12-4279-96d2-c40cedcfc225"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"polymerase chain reaction (PCR)","subitem_subject_scheme":"Other"},{"subitem_subject":"single strand conformation polymorphism (SSCP)","subitem_subject_scheme":"Other"},{"subitem_subject":"genetic mutation","subitem_subject_scheme":"Other"},{"subitem_subject":"SDS, temperature","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Single strand conformation polymorphism (SSCP) パターンに及ぼす泳動条件の影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Single strand conformation polymorphism (SSCP) パターンに及ぼす泳動条件の影響","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-04-14"},"publish_date":"2011-04-14","publish_status":"0","recid":"2381","relation_version_is_last":true,"title":["Single strand conformation polymorphism (SSCP) パターンに及ぼす泳動条件の影響"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-02T05:00:20.779913+00:00"}