@article{oai:hama-med.repo.nii.ac.jp:00002382,
 author = {前川, 真人 and 菅野, 康吉},
 issue = {1},
 journal = {生物物理化学},
 month = {Mar},
 note = {Single-strand DNA conformation polymorphism (SSCP) analysis has been used to detect alterations in relatively short DNA fragments such as genetic polymorphisms, mutations, deletions, and so on. Here we introduce other applications of SSCP analysis, using its capacity of separating alleles. A sensitive method, designated as blunt-end SSCP analysis was developed for detecting loss of heterozygosity (LOH) in cancer tissues and urine samples. The method is fluorescence-based SSCP analysis, using blunt-end DNA fragments and applied for detecting an LOH of the p53 gene. The combination of reverse transcribed-PCR and fluorescence-based SSCP analysis is proposed for the quantitative determination of ratio of mRNA molecules with homologous sequences. The procedure is applicable to a determination of expression levels of genes such as lactate dehydrogenase subunits and cyclooxygenases 1 and 2. The combination of bisulfite treatment and PCR-SSCP analysis is proposed for quantitative methylation assay. This analytic procedure can be applied to the rapid identification of methylation status in multiple samples, quantification of methylated vs. unmethylated sequences and detection of methylation heterogeneity in the amplified DNA fragments. PCR-SSCP analysis is advantageous in simple procedure with relatively high sensitivity and is applicable to any other fields.},
 pages = {5--8},
 title = {SSCP法による癌関連遺伝子の解析},
 volume = {45},
 year = {2001}
}