@article{oai:hama-med.repo.nii.ac.jp:00002412,
 author = {Hayashi, Hideharu and Miyata, Haruo and Terada, Hajime and Satoh, Hiroshi and Katoh, Hideki and Nakamura, Takurou and Kobayashi, Akira},
 issue = {5},
 journal = {Japanese Heart Journal},
 month = {Jan},
 note = {We measured [Ca2+]i of guinea pig and rat myocytes with Ca2+ waves, using fura-2 fluorescence image processing. In guinea pig myocytes, Ca2+ waves were absent during the control perfusion period, but could be induced by the addition of strophanthidin (100μM) or sodium cyanide (NaCN: 2mM) to the perfusate. The [Ca2+]i increased from the control values of 69±5nM and 46±2nM, to 263±9 (p<0.05 vs. control) nM and 225±20 (p<0.05) nM, respectively, when cells exhibited Ca2+ waves. Although 13% (16 of 121) of the rat myocytes displayed Ca2+ waves during the control perfusion, the [Ca2+]i with Ca2+ waves (56±9nM) did not differ from [Ca2+]i in the absence of Ca2+ waves (54±3nM). Ca2+ waves were induced by the perfusion with a high Ca2+ solution (24.5μM) or NaCN (2μM), and [Ca2+]i increased from the control values of 67±11nM and 74±5nM, to 231±41 (p<0.05 vs. control) nM and 266±64nM, respectively, when cells exhibited Ca2+ waves. The Ca2+ waves were abolished by the removal of extracellular Ca2+, or by the perfusion with ryanodine (10μM) or caffeine (20mM). In conclusion, it was shown that Ca2+ waves were due to oscillatory Ca2+ release and that the absolute value of [Ca2+]i is important for the appearance of Ca2+ waves in guinea pig and rat myocytes. However, some rat myocytes with a control [Ca2+]i level exhibited spontaneous Ca2+ waves during the control perfusion, showing a species difference in the susceptibility to oscillatory Ca2+ release from the sarcoplasmic reticulum.(Jpn Heart J 35: 673-682, 1994)},
 pages = {673--682},
 title = {Ca2+ Waves and Intracellular Ca2+ Concentration in Guinea Pig and Rat Myocytes},
 volume = {35},
 year = {1994}
}