{"created":"2023-06-20T15:48:08.891691+00:00","id":2671,"links":{},"metadata":{"_buckets":{"deposit":"115a6089-3b26-4c01-98a7-6e32ff2a7493"},"_deposit":{"created_by":4,"id":"2671","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"2671"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00002671","sets":["2:15:17"]},"author_link":["7804"],"item_5_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"TGFα,c-MYC，変異型CTNNB1遺伝子およびこれらの組み合わせはヌードマウスにおけるHep3B腫瘍形成に明確に作用する","subitem_alternative_title_language":"ja"}]},"item_5_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{"bibliographic_title":"Medical Journal of Osaka University","bibliographic_titleLang":"en"}]}]},"item_5_date_granted_17":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-17"}]},"item_5_degree_grantor_15":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"浜松医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13802","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_12":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_5_description_13":{"attribute_name":"学位の区分","attribute_value_mlt":[{"subitem_description":"doctoral","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_description_14":{"attribute_name":"学位の分野","attribute_value_mlt":[{"subitem_description":"医学系研究科","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hepatocellular carcinoma (HCC) is the most common hepatic tumor worldwide and is a major cause of death in many countries. Although chronic viral infections and hepatotoxic agents are the major risk factors, the molecular pathogenesis of HCC remains largely unknown. Among model mice, TGFα/c-Myc transgenic mice develop human HCC-like tumors. Whole genome sequencing of HCC tumors from patients has shown that mutations to CTNNB1 (β-catenin) are the most frequent (15.9%). So in this study, we characterized the effects of TGFα, c-MYC and mutant CTNNB1 genes on the transplantable Hep3B cells. TGFα increased the tumor number after transplanting Hep3B cells. Mutant CTNNB1 changed the characteristics of Hep3B tumor cells into a disarranged structure with loose cell-cell contacts. And co-transduction of TGFα and mutant CTNNB1 synergistically increased the growth rate, size and weight of Hep3B tumors. Unexpectedly, c-MYC overexpression in the Hep3B cells induced apoptosis. Interestingly, TGFα+mutant CTNNB1+MYC-transduced cells grew very slowly and the tumor showed transdifferentiation into a cholangial duct. These results suggest that the interaction of these three genes determines the characteristics of Hep3B tumor. Overexpression of c-MYC by a lentivirus in c-MYC-upregulated HCC tumors might be used as a molecular targeted therapy.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_dissertation_number_18":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第668号"}]},"item_5_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"大阪大学医学部","subitem_publisher_language":"ja"}]},"item_5_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0030-6169","subitem_source_identifier_type":"PISSN"}]},"item_5_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Noritake, Hidenao","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"DT_668ronbun.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"論文本文","objectType":"fulltext","url":"https://hama-med.repo.nii.ac.jp/record/2671/files/DT_668ronbun.pdf"},"version_id":"482286e2-5511-4373-80d8-74c22ab6676f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"TGFα","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"C-MYC","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mutant CTNNB1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HCC","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transdifferentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"TGFα, c-MYC, mutated CTNNB1 and their combinations act distinctly on the Hep3B tumors in nude mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"TGFα, c-MYC, mutated CTNNB1 and their combinations act distinctly on the Hep3B tumors in nude mice","subitem_title_language":"en"}]},"item_type_id":"5","owner":"4","path":["17"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-01-09"},"publish_date":"2015-01-09","publish_status":"0","recid":"2671","relation_version_is_last":true,"title":["TGFα, c-MYC, mutated CTNNB1 and their combinations act distinctly on the Hep3B tumors in nude mice"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-10-23T06:12:00.718594+00:00"}