{"created":"2023-06-20T15:49:11.769007+00:00","id":3763,"links":{},"metadata":{"_buckets":{"deposit":"b1900ad7-54d3-4081-80fb-b44001b6a846"},"_deposit":{"created_by":4,"id":"3763","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"3763"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00003763","sets":["1:11"]},"author_link":[],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"678","bibliographicPageStart":"666","bibliographicVolumeNumber":"64","bibliographic_titles":[{"bibliographic_title":"Microbiology and immunology"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Antigen-specific CD8+ T-lymphocytes (cytotoxic T-lymphocytes: CTL), as well as CD4+ T-lymphocytes (helper T-lymphocytes: Th), simultaneously play an important role in elimination of intracellular bacteria such as Mycobacterium tuberculosis and Listeria monocytogenes. Administration of T-cell epitope short peptide needs large numbers of peptides for effective vaccination due to its easily degradable nature in vivo. In this respect, biocompatible and biodegradable microparticles combined with CTL/Th-hybrid epitope long peptide (long peptide) have been used to diminish the loaded peptide degradation. The aim of this study is to develop a novel T cell-oriented vaccine against intracellular bacteria that is composed of long peptide and poly (lactic-co-glycolic acid) (PLGA) microparticles. Mouse bone marrow-derived dendritic cells (BMDCs) were loaded with L. monocytogenes listeriolysin O (LLO)-derived or ovalbumin (OVA)-derived long peptide/PLGA or other comparative antigens. The antigen-loaded BMDCs were subcutaneously injected into flank of mice twice, and then, the spleens were collected and lymphocyte proliferation and interferon-γ production were evaluated. The median diameter of PLGA spheres was 1.38 μm. Both LLO- and OVA-long peptide/PLGA showed significantly more robust CTL and Th proliferations with higher interferon-γ production than the long peptide alone or CTL and Th short peptides/PLGA vaccination. Furthermore, the LLO-long peptide/PLGA vaccination showed significantly lower bacterial burden in spleens compared with the long peptide alone or the CTL and Th short peptides/PLGA vaccination after the challenge of lethal amounts of L. monocytogenes. These results suggest that the novel vaccine taking advantages of CTL/Th-hybrid epitope long peptide and PLGA microparticle is effective for protection against intracellular bacteria.","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley and Sons "}]},"item_3_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"32786043","subitem_relation_type_select":"PMID"}}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/1348-0421.12836","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"\"This is the peer reviewed version of the following article: \"\"Development of a novel T cell-oriented vaccine using CTL/Th-hybrid epitope long peptide and biodegradable microparticles, against an intracellular bacterium, Microbiology and immunology, 64(10): 666-678, (2020)\"\", which has been published in final form at https://doi.org/10.1111/1348-0421.12836. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.\""}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-5600","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-0421","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanaka, Kazuki"}]},{"creatorNames":[{"creatorName":"Enomoto, Noriyuki"}]},{"creatorNames":[{"creatorName":"Uehara, Masahiro"}]},{"creatorNames":[{"creatorName":"Furuhashi, Kazuki"}]},{"creatorNames":[{"creatorName":"Sakurai, Shogo"}]},{"creatorNames":[{"creatorName":"Yasui, Hideki"}]},{"creatorNames":[{"creatorName":"Karayama, Masato"}]},{"creatorNames":[{"creatorName":"Hozumi, Hironao"}]},{"creatorNames":[{"creatorName":"Suzuki, Yuzo"}]},{"creatorNames":[{"creatorName":"Fujisawa, Tomoyuki"}]},{"creatorNames":[{"creatorName":"Inui, Naoki"}]},{"creatorNames":[{"creatorName":"Nakamura, Yutaro"}]},{"creatorNames":[{"creatorName":"Nagata, Toshi"}]},{"creatorNames":[{"creatorName":"Suda, Takafumi"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-08-20"}],"displaytype":"detail","filename":"Microbiol immunol.-64-666.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Microbiol immunol.-64-666.pdf","url":"https://hama-med.repo.nii.ac.jp/record/3763/files/Microbiol immunol.-64-666.pdf"},"version_id":"77e9ff89-7302-4ffe-b2c6-e50e6a1e3e59"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CTL/Th-hybrid epitope long peptide","subitem_subject_scheme":"Other"},{"subitem_subject":"Cross-presentation","subitem_subject_scheme":"Other"},{"subitem_subject":"Intracellular bacteria","subitem_subject_scheme":"Other"},{"subitem_subject":"Microparticle","subitem_subject_scheme":"Other"},{"subitem_subject":"PLGA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of a novel T cell-oriented vaccine using CTL/Th-hybrid epitope long peptide and biodegradable microparticles, against an intracellular bacterium","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of a novel T cell-oriented vaccine using CTL/Th-hybrid epitope long peptide and biodegradable microparticles, against an intracellular bacterium","subitem_title_language":"en"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-08-20"},"publish_date":"2021-08-20","publish_status":"0","recid":"3763","relation_version_is_last":true,"title":["Development of a novel T cell-oriented vaccine using CTL/Th-hybrid epitope long peptide and biodegradable microparticles, against an intracellular bacterium"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-02T05:24:40.634119+00:00"}