{"created":"2023-06-20T15:49:12.046947+00:00","id":3769,"links":{},"metadata":{"_buckets":{"deposit":"f75aba06-6e97-4913-8c48-5c4c3a7a909c"},"_deposit":{"created_by":4,"id":"3769","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"3769"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00003769","sets":["1:11"]},"author_link":[],"item_3_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Quantitation of plasma free aprepitant and its metabolite"}]},"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"428","bibliographicPageStart":"422","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"Therapeutic Drug Monitoring"}]}]},"item_3_description_10":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"雑誌掲載タイトル：Simple Liquid Chromatography?Tandem Mass Spectrometry Method for Quantitation of Total and Free Aprepitant and Its Active N-Dealkylated Metabolites in Human Plasma","subitem_description_type":"Other"}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Aprepitant, an antiemetic selective neurokinin-1 receptor antagonist, is primarily metabolized to active N-dealkylated form (ND-AP) and then converted to its carbonyl form (ND-CAP) in humans. This study developed simple liquid chromatography-tandem mass spectrometry methods using electrospray ionization (ESI) for the quantitation of plasma total and free aprepitant and its N-dealkylated metabolites and applied it to patient plasma.\nMethods: Free aprepitant and ND-AP in plasma were fractionated by centrifugal ultrafiltration. The analytes in plasma or its ultrafiltrated specimen treated by triethylamine/acetonitrile were isocratically separated on a 3-μm octadecylsilyl column with a total run time of 10 minutes and scanned with positive ion ESI.\nResults: The calibration curves of total aprepitant, ND-AP, and ND-CAP ranged from 50?2500, 20?1000, and 5?250 ng/mL, respectively, while that of free aprepitant and ND-AP ranged from 2?150 ng/mL. Their intra- and inter-assay accuracy and imprecision values were 93.5 107.7% and 94.6?103.3%, and 2.1?7.5% and 1.0?8.9%, respectively. Aprepitant and its metabolites did not exhibit any matrix effects or instabilities in plasma specimens. In cancer patients receiving oral aprepitant, the plasma concentration ranges of total aprepitant, ND-AP, and ND-CAP, and of free aprepitant and ND-AP were 137?2170, 104?928, 22.4?97.6, 8.11?60.0, and 3.53?56.0 ng/mL, respectively. The median plasma free fraction rates of aprepitant and ND-AP were 4.14% and 4.90%, respectively.\nConclusions: The present methods with acceptable analytical performance can be used to evaluate total and free aprepitant and its N-dealkylated metabolites in patient plasma.","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wolters Kluwer Health"}]},"item_3_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"32960546","subitem_relation_type_select":"PMID"}}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1097/FTD.0000000000000815","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright 2021 Wolters Kluwer Health."},{"subitem_rights":"\"\"\"This is a non-final version of an article published in final form in \"\"\"\"Therapeutic Drug Monitoring\"\"\"\", 43(3): p422-428.\"\"\""}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0163-4356","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1536-3694","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Naito, Takafumi"}]},{"creatorNames":[{"creatorName":"Suzuki, Yusuke"}]},{"creatorNames":[{"creatorName":"Shibata, Kaito"}]},{"creatorNames":[{"creatorName":"Kawakami, Junichi"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-09-22"}],"displaytype":"detail","filename":"Ther Drug Monit-43-422.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_9","mimetype":"application/pdf","url":{"label":"Ther Drug Monit-43-422.pdf","url":"https://hama-med.repo.nii.ac.jp/record/3769/files/Ther Drug Monit-43-422.pdf"},"version_id":"ba6c532b-49af-4f5a-8539-fc5ee8aab97a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"aprepitant","subitem_subject_scheme":"Other"},{"subitem_subject":"LC-MS/MS","subitem_subject_scheme":"Other"},{"subitem_subject":"metabolite","subitem_subject_scheme":"Other"},{"subitem_subject":"human plasma","subitem_subject_scheme":"Other"},{"subitem_subject":"free fraction","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Simple LC-MS/MS Methods for the Quantitation of Total and Free Aprepitant and its Active N-Dealkylated Metabolites in Human Plasma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Simple LC-MS/MS Methods for the Quantitation of Total and Free Aprepitant and its Active N-Dealkylated Metabolites in Human Plasma","subitem_title_language":"en"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-09-22"},"publish_date":"2021-09-22","publish_status":"0","recid":"3769","relation_version_is_last":true,"title":["Simple LC-MS/MS Methods for the Quantitation of Total and Free Aprepitant and its Active N-Dealkylated Metabolites in Human Plasma"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-02T07:42:13.732228+00:00"}