{"created":"2023-06-20T15:49:12.091391+00:00","id":3771,"links":{},"metadata":{"_buckets":{"deposit":"4330d624-f529-4d25-893b-e0a55b288b06"},"_deposit":{"created_by":4,"id":"3771","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"3771"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00003771","sets":["1:11"]},"author_link":[],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-07-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"529","bibliographicPageStart":"519","bibliographicVolumeNumber":"323","bibliographic_titles":[{"bibliographic_title":"Journal of Controlled Release ","bibliographic_titleLang":"en"}]}]},"item_3_description_10":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"＜参照＞出版社サイト：https://doi.org/10.1016/j.jconrel.2020.04.043　","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Tissue factor (TF), which is well known as a trigger molecule of extrinsic coagulation, is found in not only tumor cells but also in stromal cells in tumor tissues. Thus, TF is a candidate molecule to potentially enable targeting of both tumor cells and stromal cells for anti-cancer drug delivery. Herein, we prepared liposomes conjugated with the Fab’ fragment of anti-TF antibody (TF Ab- Lip) and evaluated the capability for drug delivery to stroma-rich tumors for realizing a whole tumor tissue-targetable strategy. When the targetability of TF Ab-Lip to TF-expressing KLN205 squamous tumor cells and NIH3T3 fibroblast cells were examined, TF Ab-Lip was significantly taken up into both cells compared with non-targeted liposomes. Corresponding to this result, doxorubicin-encapsulated TF Ab-Lip (TF Ab-LipDOX) showed potent cytotoxicity against KLN205 cells. In vivo experiments using KLN205 solid tumor-bearing mice indicated that TF Ab-Lip became highly accumulated and distributed widely in not only the tumor cell region but also in the stromal one in the tumor. Treatment with TF Ab-LipDOX significantly suppressed the growth of KLN205 solid tumors. Furthermore, TF Ab-Lip targetable both mouse and human TF (mhTF Ab-Lip) became distributed throughout stroma-rich human pancreatic BxPC3 tumors and the treatment of the BxPC3 tumor-bearing mice with mhTF Ab-LipDOX showed highest tumor suppressive effect. These data suggest that TF Ab-Lip could achieve effective accumulation for stroma-rich tumor treatment.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_3_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"32360306 ","subitem_relation_type_select":"PMID"}}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jconrel.2020.04.043","subitem_relation_type_select":"DOI"}}]},"item_3_relation_34":{"attribute_name":"出版社版","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jconrel.2020.04.043","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright 2020 Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"},{"subitem_rights":"This is the accepted manuscript version. The formal published version is available at \"https://doi.org/10.1016/j.jconrel.2020.04.043\".","subitem_rights_language":"en"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0168-3659","subitem_source_identifier_type":"PISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1873-4995","subitem_source_identifier_type":"EISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shimizu, Kosuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takeuchi, Yoshihito","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Otsuka, Kazuma","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mori, Tomoya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Narita, Yudai","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takasugi, Shohei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Magata, Yasuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matsumura, Yasuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Oku, Naoto","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-05"}],"displaytype":"detail","filename":"JCR-323-519.pdf","filesize":[{"value":"9.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"JCR-323-519.pdf","url":"https://hama-med.repo.nii.ac.jp/record/3771/files/JCR-323-519.pdf"},"version_id":"2cabaa99-659e-459d-88e9-c307174d2979"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"liposome","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pancreatic tumor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"targeting","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tissue factor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tumor stroma","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development of tissue factor-targeted liposomes for effective drug delivery to stroma-rich tumors","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of tissue factor-targeted liposomes for effective drug delivery to stroma-rich tumors","subitem_title_language":"en"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-10-05"},"publish_date":"2021-10-05","publish_status":"0","recid":"3771","relation_version_is_last":true,"title":["Development of tissue factor-targeted liposomes for effective drug delivery to stroma-rich tumors"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-09-21T05:04:07.525516+00:00"}