@phdthesis{oai:hama-med.repo.nii.ac.jp:00000038,
 author = {Inuzuka, Kazunori},
 month = {Jul},
 note = {浜松医科大学学位論文 医博第499号(平成20年3月17日）, doctoral, 医学系研究科, Background. Perfluorochemicals (PFC) are chemical substances that have a higher oxygen solubility under hyperbaric oxygen (HBO) pressure. This study investigated the effect of cold HBO-PFC/UW solution on preservation of rat small intestinal graft.
Methods. We manufactured an air-tight, pressure resistant tank which was made of stainless steel with high thermal conductivity. Rat ileal grafts were placed in a custom-made silicon-gum bag with UW solution, which was immersed in 5 atm HBO-PFC solution in the tank (Group P-5). The tank was placed at 4℃. We compared the ATP concentration and mucosal permeability in Group P-5 with grafts preserved in 1 atm oxygenated-PFC/UW solution (Group P-1) and simple cold storage in UW solution (Group C). Histological study was also performed.
Results. PO2 in UW solution after 48 hours preservation were 1851.6 ± 36.9, 498.5 ± 12.5, and 173.3 ± 3.4 mmHg (Group P-5, P-1 and C, respectively, mean ± SD). At that time, graft ATP concentration was significantly higher in Group P-5 compare to that in Group P-1 and Group C. Mucosal hyperpermeability as well as mucosal morphological changes were also ameliorated in the Group P-5.
Conclusion. HBO-PFC could supply a larger amount of oxygen to UW solution. Indirect measures of oxygen metabolism such as ATP content and lactate production suggested improvement in maintaining graft oxygen metabolism.},
 school = {浜松医科大学},
 title = {Effect of hyperbarically oxygenated-perfluorochemical with University of Wisconsin solution on preservation of rat small intestine using an original pressure-resistant portable apparatus},
 year = {2007}
}