{"created":"2023-06-20T15:49:14.539512+00:00","id":3816,"links":{},"metadata":{"_buckets":{"deposit":"6449e2fe-19f1-4d79-adb5-f685e869202f"},"_deposit":{"created_by":4,"id":"3816","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"3816"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00003816","sets":["1:11"]},"author_link":["11390","11389"],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"3289","bibliographicPageStart":"3279","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"Ultrasound in Medicine & Biology"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The present study aims to identify specific staining methods for acoustic histology. We compared attenuation-of-sound (AOS) images from scanning acoustic microscopy (SAM) with light microscopy (LM) images. Ethanol-fixed tissue or cytology samples and formalin-fixed surgical or autopsy specimens were examined. Nuclei, collagen, elastic fibers, and polysaccharides and various antigens, including cell surface, cytoplasmic, nuclear, and stromal substances, were observed. Samples with different fixation methods were used. Hematoxylin staining had significantly higher AOS values in accordance with staining duration. Specific staining for collagen, elastic fibers, and polysaccharides increased the AOS values of the specific substance. . Using diaminobenzidine tetrahydrochloride in NiCl2 solution as a substrate for horseradish peroxidase increased the AOS values to those suitable for acoustic immunostaining. Collagenase digestion following collagen staining decreased AOS values, reflecting collagen density and distribution. Staining with specific dyes or acoustic immunostaining enabled the histological localization of specific substances by SAM, similar to LM.","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_3_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"31563478","subitem_relation_type_select":"PMID"}}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ultrasmedbio.2019.09.003","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"},{"subitem_rights":"This is the accepted manuscript version. The formal published version is available at \"https://doi.org/10.1016/j.ultrasmedbio.2019.09.003\"."}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-5629","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1879-291X","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miura, Katsutoshi"}],"nameIdentifiers":[{"nameIdentifier":"11389","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamashita, Kanna"}],"nameIdentifiers":[{"nameIdentifier":"11390","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-26"}],"displaytype":"detail","filename":"Ultrasound Med Biol-45-3279.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"Ultrasound Med Biol-45-3279.pdf","url":"https://hama-med.repo.nii.ac.jp/record/3816/files/Ultrasound Med Biol-45-3279.pdf"},"version_id":"91fa23d6-d182-4741-b2b3-e06cc61a9bd5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Scanning acoustic microscopy","subitem_subject_scheme":"Other"},{"subitem_subject":"histology","subitem_subject_scheme":"Other"},{"subitem_subject":"cytology","subitem_subject_scheme":"Other"},{"subitem_subject":"attenuation-of-sound","subitem_subject_scheme":"Other"},{"subitem_subject":"hematoxylin","subitem_subject_scheme":"Other"},{"subitem_subject":"special staining","subitem_subject_scheme":"Other"},{"subitem_subject":"immunostaining","subitem_subject_scheme":"Other"},{"subitem_subject":"collagenase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Acoustic histology with specific dyes and antibodies","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Acoustic histology with specific dyes and antibodies"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-26"},"publish_date":"2022-01-26","publish_status":"0","recid":"3816","relation_version_is_last":true,"title":["Acoustic histology with specific dyes and antibodies"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-06-20T16:24:10.277554+00:00"}