@article{oai:hama-med.repo.nii.ac.jp:00004066,
 author = {Iwaki, Takayuki and Tomonari, Yuki and Umemura, Kazuo},
 issue = {2},
 journal = {Journal of Dairy Research},
 month = {May},
 note = {雑誌掲載タイトル：Successful lactation in Plgrkt-deficient female mice caused by a 1-bp deletion of exon4, Plasminogen (Pg) activation on the cell surfaces is an important for various (patho) physiologic condition. Plg-RKT is a cell membrane protein that binds to Pg and promotes the activation on the cell surface by Pg activators. To evaluate the role of Plg-RKT in atherosclerosis, Plgrkt gene in Ldlr-/-/Apobec1-/- was modified using in vivo CRISPR/Cas9. Synthetic RNA for Plgrkt and Cas9 complex was electroporated into the fertilized eggs in the oviducts. Plgrkt deficient mice caused by a 1-bp deletion was established. In contrast to Plgrkt-/- mice developed by conventional method, this newly developed mice did not represent lactation failure and could maintain the pups until weanling. The major obvious difference between these lines is the area of gene modification. The conventionally developed mouse possesses about 10 kb deletion of Plgrkt, which might relate to the lactation failure. Lactation failure is one of the lethal phenotypes in mammals, and analyses of causative genes are especially important for dairy industries. Further genome-wide analyses with both Plgrkt-/- mice will be helpful to find out the uncertain causative genes for lactation failure.},
 pages = {169--172},
 title = {Plgrkt-deficient female mice caused by a 1-bp deletion of exon4 can maintain colonies through sufficient lactation},
 volume = {89},
 year = {2022}
}