{"created":"2023-06-20T15:49:39.662751+00:00","id":4245,"links":{},"metadata":{"_buckets":{"deposit":"256d9b74-d7b2-44ff-8e30-b3657a79e90f"},"_deposit":{"created_by":4,"id":"4245","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"4245"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00004245","sets":["1:11"]},"author_link":["13071","13075","13072","13073","13076","13074"],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"5511","bibliographicPageStart":"5503","bibliographicVolumeNumber":"48","bibliographic_titles":[{"bibliographic_title":"Molecular Biology Reports"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background Although high doses of proton pump inhibitors can elicit an anticancer effect, this strategy may impair vascular biology. In particular, their effects on endothelial Ca2⁺ signaling and production of endothelium-derived relaxing factor (EDRF) are unknown. To this end, we investigated the effects of high dosages of omeprazole on endothelial Ca2⁺ responses and EDRF production in primary cultured porcine aortic endothelial cells.\nMethods and Results Omeprazole (10–1000μM) suppressed both bradykinin (BK)- and thapsigargin-induced endothelial Ca2⁺ response in a dose-dependent manner. Furthermore, omeprazole slightly attenuated Ca2⁺ mobilization from the endoplasmic reticulum, whereas no inhibitory effects on endoplasmic reticulum Ca2⁺-ATPase were observed. Omeprazole decreased BK-induced phosphorylation of endothelial nitric oxide synthase (eNOS) at Ser1177 and tended to decrease BK-induced nitric oxide production. Production of prostaglandin I₂ metabolites, especially 6-keto-prostaglandin 1α, also tended to be reduced by omeprazole.\nConclusion Our results are the first to indicate that high doses of omeprazole may suppress both store-operated Ca2⁺ channels and partially the G protein-coupled receptor/phospholipase C/inositol 1,4,5-triphosphate pathway, and decreased BKinduced, Ca2⁺-dependent phosphorylation of eNOS(Ser1177). Thus, high dosages of omeprazole impaired EDRF production by attenuating intracellular Ca2⁺ signaling.","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_3_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"34291395","subitem_relation_type_select":"PMID"}}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s11033-021-06561-0","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s11033-021-06561-0"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-4851","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1573-4978","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_23":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00745797","subitem_source_identifier_type":"NCID"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kamiya, Chiaki"}],"nameIdentifiers":[{"nameIdentifier":"13071","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Odagiri, Keiichi"}],"nameIdentifiers":[{"nameIdentifier":"13072","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hakamata, Akio"}],"nameIdentifiers":[{"nameIdentifier":"13073","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakurada, Ryugo"}],"nameIdentifiers":[{"nameIdentifier":"13074","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inui, Naoki"}],"nameIdentifiers":[{"nameIdentifier":"13075","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"13076","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2023-05-30"}],"displaytype":"detail","filename":"Mol.Bio.Rep.-48-5503.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Mol.Bio.Rep.-48-5503.pdf","url":"https://hama-med.repo.nii.ac.jp/record/4245/files/Mol.Bio.Rep.-48-5503.pdf"},"version_id":"a8c1704c-ec78-4287-b5d9-30bbea585ba1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"omeprazole","subitem_subject_scheme":"Other"},{"subitem_subject":"nitric oxide synthase","subitem_subject_scheme":"Other"},{"subitem_subject":"endothelial cells","subitem_subject_scheme":"Other"},{"subitem_subject":"calcium","subitem_subject_scheme":"Other"},{"subitem_subject":"endothelium-dependent relaxing factors","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Omeprazole Suppresses Endothelial Calcium Response and eNOS Ser1177 Phosphorylation in Porcine Aortic Endothelial Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Omeprazole Suppresses Endothelial Calcium Response and eNOS Ser1177 Phosphorylation in Porcine Aortic Endothelial Cells"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2023-05-30"},"publish_date":"2023-05-30","publish_status":"0","recid":"4245","relation_version_is_last":true,"title":["Omeprazole Suppresses Endothelial Calcium Response and eNOS Ser1177 Phosphorylation in Porcine Aortic Endothelial Cells"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-06-20T16:11:14.850674+00:00"}