@article{oai:hama-med.repo.nii.ac.jp:00000429,
 author = {杉本, 弘司 and SUGIMOTO, Koji and 櫻井, 信夫 and SAKURAI, Nobuo and 藤瀬, 裕 and FUJISE, Yutaka and 白沢, 春之 and SHIRASAWA, Haruyuki and 柴田, 清 and SHIBATA, Kiyoshi and 松尾, 雅子 and MATSUO, Masako and 高橋, 利枝 and TAKAHASHI, Toshie and 小森, 由美子 and KOMORI, Yumiko and 二改, 俊章 and NIKAI, Toshiaki and 杉原, 久義 and SUGIHARA, Hisayoshi},
 issue = {1},
 journal = {The journal of veterinary medical science, 日本獣醫學会会誌},
 month = {Feb},
 note = {Effective constituents for renal disease were partially purified from a herbal prescription. The efficacy on renal diseases was examined using an experimental model system of glomerular lesion in mice induced by Agkistrodon acutus venom (Ac_1-P). The extract of boiling water of the herbal prescription (P-3) was first fractionated by ether into an acidic fraction and a mixture of basic and neutral fractions. The acidic fraction was proved to be more effective and then further examined by thin layer chromatography and spectrophotometry. One of the main components was confirmed to be caffeic acid which had inhibitory effect on renal failure in mice by Ac_1-P. This effect was considered to be caused by caffeic acid inhibiting the proteolytic enzyme activity of Ac_1-P. Caffeic acid should thus have prophylactic or inhibitory effect on glomerular disease in which proteolytic enzymes may have pathogenic roles., 草薬処方剤から腎障害の改善に有効な成分の抽出分離を行ない, 精製百歩蛇毒プロテアーゼによるマウスの実験的糸球体腎炎に対する抑制効果を検討した. 草薬処方剤の熱水抽出物を通常の分別法によりエーテルで抽出し, 中性及び塩基性画分と酸性画分に分別した. 酸性画分に有効性がみられたため, さらに, 酸性画分を薄層クロマトグラフイーや分光学的手法を用いて検索した結果, 酸性画分の主要の成分のlつはカフェー酸であることが確認された. また, 市販カフェー酸は, 蛇毒プロテアーゼによるマウスの実験的腎障害に対して抑制効果を示した. これは, カフェー酸により, プロテアーゼの酵素活性の低下した結果と考えられた. カフェー酸は, 病理発生に蛋白分解酵素が関与する腎糸球体障害の予防または抑制効果があると推察された.},
 pages = {111--118},
 title = {Identification of Effective Component from a Traditional Herbal Medicine and the Inhibitory Effects on Experimental Glomerular Lesion in Mice},
 volume = {54},
 year = {1992}
}