{"created":"2023-06-20T15:45:32.743671+00:00","id":50,"links":{},"metadata":{"_buckets":{"deposit":"23d2c21b-f1ea-4f5e-bd30-aefd319684ec"},"_deposit":{"created_by":4,"id":"50","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"50"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00000050","sets":["2:15:17"]},"author_link":["155"],"item_5_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Pirh2はシグナル認識粒子受容体ベータサブユニットと結合し、ユビキチン化する","subitem_alternative_title_language":"ja"}]},"item_5_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"60","bibliographicPageStart":"53","bibliographicVolumeNumber":"29","bibliographic_titles":[{"bibliographic_title":"Biomedical Research","bibliographic_titleLang":"en"}]}]},"item_5_date_granted_17":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2008-03-17"}]},"item_5_degree_grantor_15":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"浜松医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13802","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_12":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_5_description_10":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"浜松医科大学学位論文 医博第506号(平成20年3月17日）","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_13":{"attribute_name":"学位の区分","attribute_value_mlt":[{"subitem_description":"doctoral","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_description_14":{"attribute_name":"学位の分野","attribute_value_mlt":[{"subitem_description":"医学系研究科","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Pirh2 is a RING finger type ubiquitin ligase which ubiquitylates various proteins including p53, p27Kip1, HDAC1, and ε-COP. In this study, we identified signal recognition particle receptor β subunit (SRβ), an integral membrane protein of the endoplasmic reticulum (ER), as a novel Pirh2-interacting protein by yeast two-hybrid screening. We confirmed that Pirh2 interacted with SRβ in mammalian cells. An immunofluorescent staining revealed that Pirh2 colocalized with SRβ in the ER. Pirh2 poly-ubiquitylated SRβ in an intact RING finger domain-dependent manner in vivo and in vitro. Unexpectedly, different from other Pirh2 substrates, neither overexpression of Pirh2 nor depletion of cellular Pirh2 affected SRβ protein stability. Pirh2 preferentially utilized lysine residues 6 and 29 of the ubiquitin to mediate the formation of polyubiquitin chains on SRβ. These results suggest that Pirh2 may regulate SRβ function by mediating poly-ubiquitylation of SRβ without affecting the stability of SRβ protein per se.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_dissertation_number_18":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第506号"}]},"item_5_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"バイオメディカルリサーチプレス","subitem_publisher_language":"ja"},{"subitem_publisher":"Biomedical Research Press","subitem_publisher_language":"en"}]},"item_5_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"18344599","subitem_relation_type_select":"PMID"}}]},"item_5_relation_27":{"attribute_name":"医中誌論文ID","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"2008230124","subitem_relation_type_select":"ICHUSHI"}}]},"item_5_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2220/biomedres.29.53","subitem_relation_type_select":"DOI"}}]},"item_5_relation_29":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10271/85","subitem_relation_type_select":"HDL"}}]},"item_5_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright 2008 Biomedical Research Press","subitem_rights_language":"en"}]},"item_5_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0388-6107","subitem_source_identifier_type":"PISSN"}]},"item_5_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-313X","subitem_source_identifier_type":"EISSN"}]},"item_5_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Abe, Kenji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"155","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"DT_506ronbun.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"論文本文","objectType":"fulltext","url":"https://hama-med.repo.nii.ac.jp/record/50/files/DT_506ronbun.pdf"},"version_id":"7508ee06-f455-4533-b75f-1c48f0a70b1e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Pirh2 interacts with and ubiquitylates signal recognition particle receptor β subunit","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Pirh2 interacts with and ubiquitylates signal recognition particle receptor β subunit","subitem_title_language":"en"}]},"item_type_id":"5","owner":"4","path":["17"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"50","relation_version_is_last":true,"title":["Pirh2 interacts with and ubiquitylates signal recognition particle receptor β subunit"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-10-30T04:19:40.836766+00:00"}