@phdthesis{oai:hama-med.repo.nii.ac.jp:00000054,
 author = {Hayashi, Tadataka},
 month = {Jun},
 note = {浜松医科大学学位論文 医博第515号(平成20年3月17日）, doctoral, 医学系研究科, The exact mechanism of blood vessel thrombus formation remains to be defined. Here, we introduce a new approach to probe thrombus formation in blood vessels of living animals using intravital microscopy in green fluorescent protein (GFP)-transgenic mice to simultaneously monitor platelet aggregation and procoagulant activity. To this end, GFP-expressing platelets and annexin A5 labeled with a fluorescent dye were employed to visualize and analyze platelet aggregation and markers of procoagulant activity (platelet surface phosphatidylserine, PS). Laser-induced thrombi increased and then decreased in size with time in vessels of living animals, whereas platelet surface PS initiated at the site of injury and then penetrated into the thrombus. PS-positive platelets were predominantly localized in the center of the thrombus, as was fibrin generation. The experimental system proposed here is a valuable tool not only for investigating mechanisms of thrombus formation but also to assess the efficacy of anti-thrombotic drugs within the vasculature.},
 school = {浜松医科大学},
 title = {Real-time analysis of platelet aggregation and procoagulant activity during thrombus formation in vivo},
 year = {2008}
}