{"created":"2023-06-20T15:46:06.745844+00:00","id":601,"links":{},"metadata":{"_buckets":{"deposit":"1f76e596-203f-4368-acc1-6c870fbefe06"},"_deposit":{"created_by":4,"id":"601","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"601"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00000601","sets":["1:11"]},"author_link":["1863"],"item_3_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Improved method of estimating background fluorescence intensity for intracellular free calcium ion distribution with video fluorescence microscopy"}]},"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1992-09-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"T124","bibliographicPageStart":"T119","bibliographicVolumeNumber":"41","bibliographic_titles":[{"bibliographic_title":"分析化学"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"蛍光顕微鏡と画像処理を用いた, 蛍光性カルシウム指示薬Fura-2による細胞内遊離カルシウムイオン濃度分布測定で, 細胞周辺部のカルシウムイオン濃度が異常に高濃度に推定される場合が多い.これは, 蛍光顕微鏡でしばしば見られる蛍光像のハレーションが原因である.このため, 細胞の透過光像と, カルシウムイオン分布測定時の蛍光像を重ね合わせて表示して, バックグラウンド蛍光強度と共に, ハレーション強度を指定できるようにした.更にハレーション強度以下の蛍光強度の場合, カルシウムイオン分布を表示しないようにすることで, 細胞像とよく一致した分布像を得ることが容易になった.このバックグラウンド蛍光推定法は, カルシウムイオン以外に, 同様な蛍光性指示薬が用いられるマグネシウムやナトリウムイオン, pHなどでも利用可能である.  The distribution of intracellular free calcium ion concentration ([Ca^<2+>]_i) is measured using a fluorescent calcium indicator dye and video fluorescence microscope. In many cases, the [Ca^<2+>]_i distribution around cells is unusually concentration. This well-known phenomenon is due to the halation from the fluorescent cell images. The [Ca^<2+>]_i distribution containing the halation is usually calculated by a conventional background estimation method. In the improved method, three types of background fluorescence intensity are distinguished. Two types are the same as the conventional background intensity. The third is halation intensity. The estimation of halation intensity is performed as follows. The first step is the display of superimposed fluorescent image and cell image obtained by transmitted light microscopy. The second step specifies the halation intensity by the fluorescence intensity at the edge of the cell. The [Ca^<2+>]_i distribution is calculated only from that fluorescent intensity which exceeds the halation intensity. By this improved estimation method, the [Ca^<2+>]_i distribution correspondence to the microscopic cell images could be obtaind.","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本分析化学会"}]},"item_3_relation_22":{"attribute_name":"NII論文ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"110002906448","subitem_relation_type_select":"NAID"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本分析化学会"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"宮川, 厚夫"}],"nameIdentifiers":[{"nameIdentifier":"1863","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"110002906448.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"110002906448.pdf","url":"https://hama-med.repo.nii.ac.jp/record/601/files/110002906448.pdf"},"version_id":"ecab7249-2955-41b5-b0a6-2e2db842477b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"intracellular free calcium ion","subitem_subject_scheme":"Other"},{"subitem_subject":"Fura-2","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorescence microscope","subitem_subject_scheme":"Other"},{"subitem_subject":"video image processing","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ビデオ蛍光顕微鏡を用いた細胞内遊離カルシウムイオン濃度分布測定におけるバックグラウンド蛍光の補正法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ビデオ蛍光顕微鏡を用いた細胞内遊離カルシウムイオン濃度分布測定におけるバックグラウンド蛍光の補正法","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"601","relation_version_is_last":true,"title":["ビデオ蛍光顕微鏡を用いた細胞内遊離カルシウムイオン濃度分布測定におけるバックグラウンド蛍光の補正法"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-02T05:48:27.449337+00:00"}