@phdthesis{oai:hama-med.repo.nii.ac.jp:00000802,
 author = {Otsuka, Atsushi},
 month = {Jun},
 note = {浜松医科大学学位論文 医博論第456号(平成20年7月18日）, doctoral, 医学系研究科, We investigated the presence of β-adrenoceptor subtypes in human urinary bladder urothelium and examined whether β-adrenoceptors in the urothelium modulate the relaxation responses of isolated human detrusor strips to a β-adrenoceptor agonist. Expression of β1-, β2- and β3-adrenoceptor mRNA in urothelium and detrusor smooth muscle was determined by reverse transcription-polymerase chain reaction (RT-PCR), and the distribution of β1-, β2- and β3-adrenoceptors in human urinary bladder urothelium was examined by immunohistochemistry. Paired human longitudinal detrusor strips with and without an intact urothelium were suspended in organ baths to construct concentration-response curves to isoproterenol. The possible involvement of urothelium-derived nitric oxide (NO) in this response was examined in additional experiments with urothelium-intact strips in the presence of NG-nitro-L-arginine methylester (L-NAME). Results confirmed the expression of β1-, β2- and β3-adrenoceptors in the human urinary bladder urothelium. Further, the presence of the urothelium caused a parallel rightward shift of the concentration-response curve to isoproterenol, with a significant reduction in potency (pEC50). L-NAME failed to exert any significant effect on the relaxation response to isoproterenol in the urothelium-intact strips. These results confirm the presence of β1-, β2- and β3-adrenoceptors in human urinary bladder urothelium. Further, they suggest that urothelial β-adrenoceptors induce the release of a urothelium-derived factor which inhibits the β-adrenoceptor agonist-induced relaxation of the human detrusor smooth muscle, and that this inhibitory mechanism might not involve NO.},
 school = {浜松医科大学},
 title = {Expression and functional role of β-adrenoceptors in the human urinary bladder urothelium},
 year = {2008}
}