{"created":"2023-06-20T15:46:18.133416+00:00","id":802,"links":{},"metadata":{"_buckets":{"deposit":"93776026-8384-4137-b3e2-4e7b66c61a0d"},"_deposit":{"created_by":4,"id":"802","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"802"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00000802","sets":["2:15:17"]},"author_link":["2714"],"item_5_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ヒト膀胱上皮におけるβ-アドレナリン受容体の発現と機能的役割","subitem_alternative_title_language":"ja"}]},"item_5_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4-6","bibliographicPageEnd":"481","bibliographicPageStart":"473","bibliographicVolumeNumber":"377","bibliographic_titles":[{"bibliographic_title":"Naunyn-Schmiedeberg's Archives of Pharmacology","bibliographic_titleLang":"en"}]}]},"item_5_date_granted_17":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2008-07-18"}]},"item_5_degree_grantor_15":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"浜松医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"13802","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_12":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_5_description_10":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"浜松医科大学学位論文 医博論第456号(平成20年7月18日）","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_13":{"attribute_name":"学位の区分","attribute_value_mlt":[{"subitem_description":"doctoral","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_description_14":{"attribute_name":"学位の分野","attribute_value_mlt":[{"subitem_description":"医学系研究科","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We investigated the presence of β-adrenoceptor subtypes in human urinary bladder urothelium and examined whether β-adrenoceptors in the urothelium modulate the relaxation responses of isolated human detrusor strips to a β-adrenoceptor agonist. Expression of β1-, β2- and β3-adrenoceptor mRNA in urothelium and detrusor smooth muscle was determined by reverse transcription-polymerase chain reaction (RT-PCR), and the distribution of β1-, β2- and β3-adrenoceptors in human urinary bladder urothelium was examined by immunohistochemistry. Paired human longitudinal detrusor strips with and without an intact urothelium were suspended in organ baths to construct concentration-response curves to isoproterenol. The possible involvement of urothelium-derived nitric oxide (NO) in this response was examined in additional experiments with urothelium-intact strips in the presence of NG-nitro-L-arginine methylester (L-NAME). Results confirmed the expression of β1-, β2- and β3-adrenoceptors in the human urinary bladder urothelium. Further, the presence of the urothelium caused a parallel rightward shift of the concentration-response curve to isoproterenol, with a significant reduction in potency (pEC50). L-NAME failed to exert any significant effect on the relaxation response to isoproterenol in the urothelium-intact strips. These results confirm the presence of β1-, β2- and β3-adrenoceptors in human urinary bladder urothelium. Further, they suggest that urothelial β-adrenoceptors induce the release of a urothelium-derived factor which inhibits the β-adrenoceptor agonist-induced relaxation of the human detrusor smooth muscle, and that this inhibitory mechanism might not involve NO.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_dissertation_number_18":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙第456号"}]},"item_5_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer","subitem_publisher_language":"en"}]},"item_5_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"18311486","subitem_relation_type_select":"PMID"}}]},"item_5_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s00210-008-0274-y","subitem_relation_type_select":"DOI"}}]},"item_5_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright Springer-Verlag 2008","subitem_rights_language":"en"}]},"item_5_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0028-1298","subitem_source_identifier_type":"PISSN"}]},"item_5_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1432-1912","subitem_source_identifier_type":"EISSN"}]},"item_5_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Otsuka, Atsushi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"DT_ron456ronbun.pdf","filesize":[{"value":"501.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"論文本文","objectType":"fulltext","url":"https://hama-med.repo.nii.ac.jp/record/802/files/DT_ron456ronbun.pdf"},"version_id":"9ef10e36-9211-4e94-ad01-f6fe1bef3112"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"human urothelium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"β-adrenoceptor agonist","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mucosa","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"β3-adrenoceptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bladder","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nitric oxide","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Expression and functional role of β-adrenoceptors in the human urinary bladder urothelium","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression and functional role of β-adrenoceptors in the human urinary bladder urothelium","subitem_title_language":"en"}]},"item_type_id":"5","owner":"4","path":["17"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"802","relation_version_is_last":true,"title":["Expression and functional role of β-adrenoceptors in the human urinary bladder urothelium"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-10-30T04:20:09.752841+00:00"}