{"created":"2023-06-20T15:46:18.415289+00:00","id":806,"links":{},"metadata":{"_buckets":{"deposit":"3d0859bb-a3b5-4789-a10c-6196ee0d8080"},"_deposit":{"created_by":4,"id":"806","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"806"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00000806","sets":["1:11"]},"author_link":["2722","2723","2724","2725"],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-01-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"478","bibliographicPageStart":"470","bibliographicVolumeNumber":"113","bibliographic_titles":[{"bibliographic_title":"Blood","bibliographic_titleLang":"en"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We analyzed the secretory dynamics of tissue plasminogen activator (tPA) in EA.hy926 cells, an established vascular endothelial cell (VEC) line producing GFP-tagged tPA, using total internal reflection fluorescence (TIR-F) microscopy. tPA-GFP was detected in small granules in EA.hy926 cells, the distribution of which was indistinguishable from intrinsically expressed tPA. Its secretory dynamics were unique, with prolonged (>5min.) retention of the tPA-GFP on the cell surface, appearing as fluorescent spots in two-thirds of the exocytosis events. The rapid disappearance (mostly by 250ms) of a domain-deletion mutant of tPA-GFP possessing only the signal peptide and catalytic domain indicates that the amino-terminal heavy chain of tPA-GFP is essential for binding to the membrane surface. The addition of PAI-1 dose-dependently facilitated the dissociation of membrane-retained tPA and increased the amounts of tPA-PAI-1 high molecular weight complexes in the medium. Accordingly, suppression of PAI-1 synthesis in EA.hy926 cells by siRNA prolonged the dissociation of tPA-GFP, whereas a catalytically inactive mutant of tPA-GFP not forming complexes with PAI-1 remained on the membrane even after PAI-1 treatment. Our results provide new insights into the relationship between exocytosed, membrane-retained tPA and PAI-1, which would modulate cell surface-associated fibrinolytic potential.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society of Hematology","subitem_publisher_language":"en"}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1182/blood-2008-03-144279","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright 2009 by American Society of Hematology","subitem_rights_language":"en"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00064971","subitem_source_identifier_type":"ISSN"}]},"item_3_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"15280020","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suzuki, Yuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2722","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mogami, Hideo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2723","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ihara, Hayato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2724","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Urano, Tetsumei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2725","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"blood-113-470.pdf","filesize":[{"value":"533.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"blood-113-470.pdf","url":"https://hama-med.repo.nii.ac.jp/record/806/files/blood-113-470.pdf"},"version_id":"b50ad976-f846-4677-9ed0-b232a94ea26a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"VON-WILLEBRAND-FACTOR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"EUGLOBULIN CLOT LYSIS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"WEIBEL-PALADE BODIES","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PROTEIN-KINASE-C","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"T-PA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MYOCARDIAL-INFARCTION","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PLASMA-LEVELS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ACUTE RELEASE","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"EXOCYTOSIS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FIBRINOLYSIS","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells","subitem_title_language":"en"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"806","relation_version_is_last":true,"title":["Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-02T03:47:31.448864+00:00"}