{"created":"2023-06-20T15:46:18.895113+00:00","id":813,"links":{},"metadata":{"_buckets":{"deposit":"fba1df32-a78b-4b7c-9173-ae2761ec7cba"},"_deposit":{"created_by":4,"id":"813","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"813"},"status":"published"},"_oai":{"id":"oai:hama-med.repo.nii.ac.jp:00000813","sets":["1:11"]},"author_link":["2758","2759","2760"],"item_3_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"198","bibliographicPageStart":"187","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"ZOOLOGICAL SCIENCE","bibliographic_titleLang":"en"}]}]},"item_3_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Newly laid eggs of many insect species store maternal ecdysteroids as physiologically inactive phosphoric esters. In the silkworm Bombyx mori, we previously reported the presence of a specific enzyme, called ecdysteroid-phosphate phosphatase (EPPase), which catalyzes the dephosphorylation of ecdysteroid-phosphates to increase the amount of free ecdysteroids during early embryonic development. In this study, we demonstrated that (1) EPPase is found in the cytosol of yolk cells, (2) ecdysteroid-phosphates are localized in yolk granules, being bound to the yolk protein vitellin (Vn), and (3) Vn-bound ecdysteroid-phosphates are scarcely hydrolyzed by EPPase, although free ecdysteroid-phosphates are completely hydrolyzed by EPPase. Thus, we investigated the mechanism by which ecdysteroid-phosphates dissociate from the Vn-ecdysteroid-phosphate complex, and indicated that the acidification of yolk granules causes the dissociation of ecdysteroid-phosphates from the Vn-ecdysteroid-phosphate complex and thereby ecdysteroid-phosphates are released from yolk granules into the cytosol. Indeed, the presence of vacuolar-type proton-translocating ATPase in the membrane fraction of yolk granules was also verified by Western blot analysis. Our experiments revealed that Vn functions as a reservoir of maternal ovarian ecdysteroid-phosphates as well as a nutritional source during embryonic development. This is the first report showing the biochemical mechanism by which maternal Vn-bound ecdysteroid-phosphates function during early embryonic development.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Zoological Society of Japan","subitem_publisher_language":"en"}]},"item_3_relation_28":{"attribute_name":"出版社DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2108/zsj.22.187","subitem_relation_type_select":"DOI"}}]},"item_3_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright 2005 Zoological Society of Japan","subitem_rights_language":"en"}]},"item_3_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"02890003","subitem_source_identifier_type":"ISSN"}]},"item_3_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamada, Ryouichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2758","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamahama, Yumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2759","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sonobe, Haruyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2760","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-08-27"}],"displaytype":"detail","filename":"ZOOLOGICAL SCIENCE-22-187.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ZOOLOGICAL SCIENCE-22-187.pdf","url":"https://hama-med.repo.nii.ac.jp/record/813/files/ZOOLOGICAL SCIENCE-22-187.pdf"},"version_id":"d368b59b-18ff-4843-9d7a-1a10e7c5de59"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ecdysteroids","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphatase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"V-ATPase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"vitellin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"embryonic development","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Release of Ecdysteroid-Phosphates from Egg Yolk Granules and Their Dephosphorylation during Early Embryonic Development in Silkworm, Bombyx mori","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Release of Ecdysteroid-Phosphates from Egg Yolk Granules and Their Dephosphorylation during Early Embryonic Development in Silkworm, Bombyx mori","subitem_title_language":"en"}]},"item_type_id":"3","owner":"4","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-08-27"},"publish_date":"2013-08-27","publish_status":"0","recid":"813","relation_version_is_last":true,"title":["Release of Ecdysteroid-Phosphates from Egg Yolk Granules and Their Dephosphorylation during Early Embryonic Development in Silkworm, Bombyx mori"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-08-02T03:47:36.326775+00:00"}