| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
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|
タイトル |
TISSUE DAMAGE BY DIQUAT REVEALED BY ASCORBATE FREE RADICAL FORMATION |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Diquat |
| キーワード |
|
|
主題 |
Ascorbate radical |
| キーワード |
|
|
主題 |
Electron spin resonance |
| キーワード |
|
|
主題 |
ODS Rat |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他のタイトル |
|
|
その他のタイトル |
アスコルベートラジカル産生からみたジクワットによる組織の損傷 |
| 著者 |
Minakata, Kayoko
Suzuki, Osamu
Suzuki, Masako
Saito, Shin-ichi
Harada, Naoko
|
| 書誌情報 |
ja : 法中毒
en : Japanese journal of forensic toxicology
巻 17,
号 3,
p. 187-194,
発行日 1999-09-15
|
| 出版者 |
|
|
出版者 |
日本法中毒学会 |
|
言語 |
ja |
| 権利 |
|
|
権利情報 |
Copyright 日本法中毒学会 |
| 権利 |
|
|
権利情報 |
本文データは日本法中毒学会の許諾のもと掲載しています。 |
| 抄録 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
In vitro formation of ascorbate radical (A・-) has been demonstrated for the supernatant fractions of homogenates of rat spleen, lung, kidney, liver, heart and testis after chronic administration of diquat (DQ) to the animals. Male ODS rats, unable to synthesize ascorbic acid (AH2) , were fed with or without 587 ppm DQ in the diet for 14 days. The supernatants of the tissue homogenates were kept at 4℃ for various intervals, and their A・- levels were measured by an electron spin resonance (ESR) method. At the beginning of the measurements, A・- was detected only in the supernatant fractions of the spleen and lung of both untreated and treated rats. In the spleen supernatant of the treated rat, A・- level increased to twice the initial level at 2 h and decreased to 0.2-0.6-fold of the initial level at 24 h, whereas that in control rat spleen supernatant increased slowly to 1.1 times the initial level at 3 h and decreased to 0.7-fold at 24 h. The maximum A・- level in the treated rat lung supernatant was only 1. 2 times that of the control. In the supernatant fractions of other organs of both untreated and treated rats, such as the kidney, liver, heart and testis, A・- was not detected initially but became detectable according to time; in the treated kidney and liver supernatant fractions, the maximum A・- levels were nearly twice those of controls, like in the spleen homogenate. For heart and testis homogenates, no significant differences were observed between test and control samples. The level of DQ, known・to be metabolized in the liver, was highest in the spleen followed by the kidney. Direct addition of DQ to control organ homogenates, however, neither enhanced nor quenched A・- formation. Therefore, the formation of A・- in tissue supernatant of the treated rats may be due to induction of A・- forming oxidative enzyme(s) and/or due to formation of reactive oxygen species during chronic exposure of the animals to DQ. Our results also indicate that A・- is one of the good indicators of tissue damages. |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
09159606 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
FT-17-187.pdf (359.3 kB)
