| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
|
|
タイトル |
SIMPLE ANALYSIS OF ACETAMINOPHEN IN HUMAN PLASMA BY SOLID-PHASE MICROEXTRACTION AND GAS CHROMATOGRAPHY |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Solid-phase microextraction (SPME) |
| キーワード |
|
|
主題 |
Acetaminophen |
| キーワード |
|
|
主題 |
2-Acetamidophenol |
| キーワード |
|
|
主題 |
Gas chromatography |
| キーワード |
|
|
主題 |
Flame thermionic detection |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他のタイトル |
|
|
その他のタイトル |
固相マイクロ抽出法を用いた血漿中アセトアミノフェンの簡便ＧＣ分析 |
| 著者 |
Hamajima, Makoto
Ishida, Saori
Hirata, Yukari
Hirata, Kouichi
Watanabe, Kanako
Seno, Hiroshi
Ishii, Akira
|
| 書誌情報 |
ja : 法中毒
en : Japanese journal of forensic toxicology
巻 22,
号 1,
p. 22-26,
発行日 2004-01-31
|
| 出版者 |
|
|
出版者 |
日本法中毒学会 |
|
言語 |
ja |
| 権利 |
|
|
権利情報 |
Copyright 日本法中毒学会 |
| 権利 |
|
|
権利情報 |
本文データは日本法中毒学会の許諾のもと掲載しています。 |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
We have developed a simple method for extraction of acetaminophen (paracetamol) in human plasma by solid-phase microextraction (SPME) using 2-acetamidophenol as internal standard (IS). After heating a vial containing a plasma sample with acetaminophen and IS at 65℃ for 30 min in the presence of Na2SO4 and NaHCO3 solution, a polyacrylate-coated fiber was exposed to the liquid-phase to allow adsorption of acetaminophen and IS. The fiber needle was then injected into a capillary gas chromatograph for analysis. Although the extraction efficiencies of acetaminophen were 0.12-0.13%, the calibration curve showed good linearity in the range of 2.5-200 μg/ml; intra- and inter-day assay coefficients of variation were 5.8 and 13.6% for 100 μg/ml acetaminophen, respectively. The detection limit was about 0.5 μg/ml. Acetaminophen could be actually measured by this method for plasma specimens of volunteers, who had ingested the drug. The above results show that the present SPME method is recommendable for analysis of acetaminophen in forensic toxicology and clinical pharmacology, because of its simplicity and reliability. |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09159606 |
| 著者版フラグ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
FT-22-22.pdf (411.0 kB)
