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Cytokine responses of intraepithelial lymphocytes are regulated by histamine H2 receptor
http://hdl.handle.net/10271/1895
http://hdl.handle.net/10271/18952007c9a7-6859-47ad-bb8c-ec1c32c06c01
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
論文本文 (380.2 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2013-08-27 | |||||||
| タイトル | ||||||||
| タイトル | Cytokine responses of intraepithelial lymphocytes are regulated by histamine H2 receptor | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | histamine | |||||||
| キーワード | ||||||||
| 主題 | IEL | |||||||
| キーワード | ||||||||
| 主題 | histamine H2 receptor | |||||||
| キーワード | ||||||||
| 主題 | IFN-γ, | |||||||
| キーワード | ||||||||
| 主題 | TCRαβ | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| その他のタイトル | ||||||||
| その他のタイトル | 腸管上皮間リンパ球のサイトカイン応答はヒスタミンH2受容体による制御を受ける | |||||||
| 著者 |
Takagaki, Kosuke
× Takagaki, Kosuke
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| 書誌情報 |
en : Journal of Gastroenterology 巻 44, 号 4, p. 285-296, 発行日 2009-04-01 |
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| 出版者 | ||||||||
| 出版者 | Springer | |||||||
| 言語 | en | |||||||
| 権利 | ||||||||
| 言語 | en | |||||||
| 権利情報 | Copyright Springer 2009 | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Backgrounds. Histamine participates in the immune regulation of several gastrointestinal diseases. However, the effect of histamine on intestinal intraepithelial lymphocytes (IELs), the front line of intestinal mucosal immune system, is not well-understood. We examined whether histamine has a direct effect on cytokine production by IELs and the involvement of histamine receptor subtypes. Methods. Murine IELs were activated by PMA plus ionomycin with/without histamine. Secreted cytokines were measured and compared with those of splenocytes. Intracellular cytokines were detected by flow cytometory. Expression of histamine receptor subtypes in IELs was examined by RT-PCR. Results. Histamine H1 receptor (H1R), H2R, and H4R, but not H3R mRNA were expressed on IELs. Histamine significantly decreased Th1-cytokine (IFN-γ, TNF-α, and IL-2) and also IL-4 production in IELs as well as splenocytes. The selective H2R antagonist famotidine, but not the H1R antagonist pyrilamine nor the H3R/H4R antagonist thioperamide, competes with the inhibitory effect of histamine on these cytokine production in IELs. These suppressive effects of histamine were mimicked by a selective H2R/H4R agonist dimaprit. Further, these suppressive effects of histamine for Th1-cytokine and IL-4 did not accompany with the enhancement of IL-10 production nor IL-10 mRNA level in IELs. Intracellular cytokine analysis revealed that the number of IFN-γ-producingαβ T cells was significantly reduced by histamine in IELs. Conclusions. Histamine has a direct suppressive effect on IEL-derived cytokines via H2R, which would have a crucial role in the suppression of local immunoregulation in the intestinal epithelium. | |||||||
| 言語 | en | |||||||
| 注記 | ||||||||
| 内容記述 | 浜松医科大学学位論文 医博第549号(平成21年3月18日） | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（医学） | |||||||
| 学位の区分 | ||||||||
| 内容記述 | doctoral | |||||||
| 学位の分野 | ||||||||
| 内容記述 | 医学系研究科 | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 13802 | |||||||
| 学位授与機関名 | 浜松医科大学 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2009-03-18 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第549号 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 0944-1174 | |||||||
| EISSN | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 1435-5922 | |||||||
| PubMed番号 | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 19277450 | |||||||
| 出版社DOI | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1007/s00535-009-0019-9 | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
