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UBL3 interaction with α-synuclein is downregulated by silencing MGST3
http://hdl.handle.net/10271/0002000204
http://hdl.handle.net/10271/00020002043c2a471e-56ee-46c1-9ca1-c4cd27faac6a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
論文本文 (2.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2024-09-05 | |||||||
| タイトル | ||||||||
| タイトル | UBL3 interaction with α-synuclein is downregulated by silencing MGST3 | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | ubiquitin-like 3 | |||||||
| キーワード | ||||||||
| 主題 | α-synuclein | |||||||
| キーワード | ||||||||
| 主題 | microsomal glutathione s-transferase 3 | |||||||
| キーワード | ||||||||
| 主題 | oxidative stress | |||||||
| キーワード | ||||||||
| 主題 | hydrogen peroxide | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| その他のタイトル | ||||||||
| その他のタイトル | UBL3とα-シヌクレインの相互作用は、MGST3のサイレンシングによってダウンレギュレーションされる | |||||||
| 著者 |
Zhang, Hengsen
× Zhang, Hengsen
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| 書誌情報 |
en : Biomedicines 巻 11, 号 9, p. 2491, ページ数 11, 発行日 2023-09-08 |
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| 出版者 | ||||||||
| 出版者 | MDPI | |||||||
| 言語 | en | |||||||
| 権利 | ||||||||
| 言語 | en | |||||||
| 権利情報 | (C) 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Ubiquitin-like 3 (UBL3) is a membrane-anchored protein that plays a crucial role in sorting proteins into small extracellular vesicles. Aggregations of alpha-synuclein (α-syn) are associated with the pathology of neurodegenerative diseases such as Parkinson’s disease. Recently, the interaction between UBL3 and α-syn was discovered, with potential implications in clearing excess α-syn from neurons and its role in disease spread. However, the regulator that can mediate the interaction between UBL3 and α-syn remains unclear. In this study, using the split gaussian luciferase complementation assay and RNA interference technology, we identified that QSOX2, HTATIP2, UBE3C, MGST3, NSF, HECTD1, SAE1, and ATG3 were involved in downregulating the interaction between UBL3 and α-syn. Notably, silencing MGST3 had the most significant impact. Immunocytochemistry staining confirmed the impact of MGST3 silencing on the co-localization of UBL3 and α-syn in cells. MGST3 is a part of the antioxidant system, and silencing MGST3 is believed to contribute to oxidative stress. We induced oxidative stress with hydrogen peroxide, observing its effect on the UBL3-α-syn interaction, and showing that 800 μM of H₂O₂ downregulated this interaction. In conclusion, silencing MGST3 downregulates the interaction between UBL3 and α-syn. | |||||||
| 言語 | en | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（医学） | |||||||
| 学位の区分 | ||||||||
| 内容記述 | doctoral | |||||||
| 学位の分野 | ||||||||
| 内容記述 | 医学系研究科 | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 13802 | |||||||
| 学位授与機関名 | 浜松医科大学 | |||||||
| 学位授与機関名 | Hamamatsu University School of Medicine | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2023-09-22 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第958号 | |||||||
| EISSN | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 2227-9059 | |||||||
| PubMed番号 | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 37760932 | |||||||
| 出版社DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.3390/biomedicines11092491 | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
