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DESI-IMS coupled with triple quadrupole mass spectrometry demonstrated the capability of NMN imaging in Shelfordella lateralis tissue
http://hdl.handle.net/10271/0002000314
http://hdl.handle.net/10271/00020003144e92ba10-f75a-4647-b349-aa00028a3796
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
論文本文 (1.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2025-02-10 | |||||||
| タイトル | ||||||||
| タイトル | DESI-IMS coupled with triple quadrupole mass spectrometry demonstrated the capability of NMN imaging in Shelfordella lateralis tissue | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | IMS | |||||||
| キーワード | ||||||||
| 主題 | NMN | |||||||
| キーワード | ||||||||
| 主題 | DESI-TQ | |||||||
| キーワード | ||||||||
| 主題 | DESI-Q-TOF | |||||||
| キーワード | ||||||||
| 主題 | UPLC-MS/MS | |||||||
| キーワード | ||||||||
| 主題 | Shelfordella lateralis | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| その他のタイトル | ||||||||
| その他のタイトル | DESI-IMS とトリプル四重極質量分析計により可能となったShelfordella lateralis組織におけるNMNイメージング | |||||||
| 著者 |
Tamannaa, Zinat
× Tamannaa, Zinat
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| 書誌情報 |
en : Medical Mass Spectrometry 巻 8, 号 1, p. 2-10, 発行日 2024-06-25 |
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| 出版者 | ||||||||
| 出版者 | 日本医用マススペクトル学会 | |||||||
| 言語 | ja | |||||||
| 出版者 | ||||||||
| 出版者 | Japanese Society for Biomedical Mass Spectrometry | |||||||
| 言語 | en | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Imaging mass spectrometry (IMS) has become an indispensable analytical tool for visualizing molecular distributions in biological tissues. However, the detection of many important biomolecules, such as nicotinamide mononucleotide (NMN) in tissue by IMS is still challenging due to their lower abundance. Although the sensitivity of widely used IMS instruments, desorption electrospray ionization (DESI) utilizing a time-of-flight (TOF) mass analyzer, is not sufficient to detect NMN in tissues, the recently developed DESI coupled with a triple-quadrupole (TQ) provides about ten times higher sensitivity than the DESI-Q-TOF. In this study, we first screened six insect species for NMN content using ultra-performance liquid-chromatography–tandem mass spectrometry (UPLC-MS/MS). Interestingly, Shelfordella lateralis showed significantly higher NMN content (15.5mg/100g of dry weight) than five other insect species used in this study. After screening, we tried to observe the distribution of NMN in S. lateralis using both DESI-Q-TOF and DESI-TQ, and only the DESI-TQ successfully detected the NMN in S. lateralis. DESI-TQ revealed prominent distributions of NMN in the abdomen of S. lateralis in selective reaction monitoring mode. To the best of our knowledge, this is the first report of NMN imaging in animal tissue applying IMS. Our study will contribute to the extended application of IMS for NMN imaging in mammalian tissues in the future. Furthermore, NMN imaging may help researchers to study nicotinamide adenine dinucleotide (NAD+) metabolism in living organisms, which has significant implications for medical research and diagnosis. | |||||||
| 言語 | en | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（医学） | |||||||
| 学位の区分 | ||||||||
| 内容記述 | doctoral | |||||||
| 学位の分野 | ||||||||
| 内容記述 | 医学系研究科 | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 13802 | |||||||
| 学位授与機関名 | 浜松医科大学 | |||||||
| 学位授与機関名 | Hamamatsu University School of Medicine | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2024-02-14 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第965号 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 2432-7441 | |||||||
| EISSN | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 2432-745X | |||||||
| NII書誌ID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA12788254 | |||||||
| 出版社DOI | ||||||||
| 関連タイプ | isVersionOf | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.24508/mms.2024.06.001 | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
