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A Rapid and Easy Electrophoretic Method for Detecting Biochemical Loci of Rat (Rattus norvegices)
http://hdl.handle.net/10271/2054
http://hdl.handle.net/10271/2054f27ea6a4-113e-453a-9915-4b4f8859487a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ExpAnim-45_2-189.pdf (1.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2013-08-27 | |||||||||
| タイトル | ||||||||||
| タイトル | A Rapid and Easy Electrophoretic Method for Detecting Biochemical Loci of Rat (Rattus norvegices) | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | biochemical gene | |||||||||
| キーワード | ||||||||||
| 主題 | electrophoresis | |||||||||
| キーワード | ||||||||||
| 主題 | rat | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Nishikawa, Tetsu
× Nishikawa, Tetsu
× Nishimura, Masahiko
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| 書誌情報 |
Experimental Animals 巻 45, 号 2, p. 189-194, 発行日 1996 |
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| 出版者 | ||||||||||
| 出版者 | 日本実験動物学会 | |||||||||
| 権利 | ||||||||||
| 権利情報 | © 2003 Japanese Association for Laboratory Animal Science | |||||||||
| 権利 | ||||||||||
| 権利情報 | 本文データは学協会の許諾に基づきJ-STAGEから複製したものである | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Methods for electrophoresis for the analysis of biochemical marker genes, which are used widely for genetic monitoring of inbred straines of rats, have been complicated by the variation of the gel and electrode buffer and electrophoretic conditions with the enzyme or the protein to be examined. To simplify the methods, we performed electrophoresis under fixed conditions of 200 V and 30 min using cellulose acetate membrane as the gel and veronal solution as the gel and electrode buffer. Good results were obtained concerning 12 loci, namely, Amy1, Cs1, Esl, Es2, Es3, Es4, Fh1, Gc, Hbb, Ldr1, Mup1, and Svp1. This method was applied to 8 inbred strains of rats and confirmed to be practical. | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 13411357 | |||||||||
| EISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 18817122 | |||||||||
| 出版社DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1538/expanim.45.189 | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
