| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
|
|
タイトル |
抗ヒトアセチル化乳酸脱水素酵素B4抗体の性状: 非修飾乳酸脱水素酵素Bサブユニットとの反応性 |
|
言語 |
ja |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題 |
anti-lactate dehydrogenase B4 antibody |
| キーワード |
|
|
主題 |
soluble immune complex |
| キーワード |
|
|
主題 |
Heterogeneity index |
| キーワード |
|
|
主題 |
anti-acetylated lactate dehydrogenase antibody |
| キーワード |
|
|
主題 |
lactate dehydrogenase isoenzyme |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
須藤, 加代子
河野, 弘明
前川, 真人
神田, 進司
菅野, 剛史
|
| 書誌情報 |
生物物理化学
巻 28,
号 3,
p. 177-182,
発行日 1984-06-30
|
| 出版者 |
|
|
出版者 |
日本電気泳動学会 |
| 権利 |
|
|
権利情報 |
日本電気泳動学会 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
An anti-acetylated lactate dehydrogenase (LD) B4 anti-serum was prepared from a New Zealand white male rabbit. This anti-serum formed soluble LD and antibody complexes with non-acetylated LD B4 molecules without inhibiting the LD activity. This property was also found in other LD isoenzymes containing B subunit in their molecules. Changes of electrophoretic mobility towards the cathodic side were observed in these LD antibody complexes, and free and bound LD isoenzymes were completely separated and could be quantitated by densitometric analysis. These soluble complexes were completely precipitated by the addition of anti-rabbit immunoglobulin G and the free LD activity was clearly measured by the assay of remaining activity in the centrifugal supernatant. Employing these two separating methods, i. e., the electrophoretic and the immunological method, the equilibrium constant (K) and the heterogeneity index (a) of the antibody for each LD isoenzyme were measured by Sips plot analysis. The antibody showed a high affinity to LD isoenzymes and a different heterogeneity index for each LD isoenzyme. These findings should be useful for the analysis of enzyme linked immunoglobulin appearing in human sera. |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00319082 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13499785 |
| 出版社DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.2198/sbk.28.177 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
Seibutubuturi28_3-177.pdf (3.1 MB)
