| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
|
|
タイトル |
Pseudocholinesterase変異のHill numberによる解析 |
|
言語 |
ja |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題 |
pseudocholinesterase |
| キーワード |
|
|
主題 |
pseudocholinesterase variants |
| キーワード |
|
|
主題 |
atypical variant |
| キーワード |
|
|
主題 |
silent variant |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
真鍋, 恵子
真鍋, 満久
須藤, 加代子
菅野, 剛史
|
| 書誌情報 |
生物物理化学
巻 26,
号 6,
p. 457-462,
発行日 1982-12-25
|
| 出版者 |
|
|
出版者 |
日本電気泳動学会 |
| 権利 |
|
|
権利情報 |
日本電気泳動学会 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The physico-chemical and kinetical properties of abnormal pseudocholinesterase (p-ChE) in two families (J. T. and K. K.) were studied and discussed. The p-ChE levels of the patient J. T. an d K. K. were 10% and 3% of the lower limit of normal ranges, respectively. The p-ChE of patient J.T . was classified as homozygote for atypical gene by the inhibition studies. However, the p-ChE of K. K. showed much larger Km values for several substrate s than normal controls and the Hill's number of the patient's p-ChE was 1. 10, though the v alues less than 1.0 were usually obtained from normal control. Therefore, the p-ChE of K. K. have not the allosteric property with negative cooperativity, as was the nature of normal one. Further studies by isoelectric focusing and slab PAG electrophoresis revealed the traces of the activity of p-ChE in the patient J.T ., but any activity band was not detected in the patient K. K. These results indicated that the patient J. T. was homozygote of atypical gene and that the patient K. K. was hom ozygote for silent gene. It was suggested that the analysis of Hill's number, additional to the c onventional inhibition studies, might be useful for the detection of the silent gene of p-ChE in deta ils. |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00319082 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13499785 |
| 出版社DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.2198/sbk.26.457 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
Seibutubuturi26_6-457.pdf (4.3 MB)
