| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
|
|
タイトル |
酵素結合性免疫グロブリン検出への免疫固定法の応用 |
|
言語 |
ja |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題 |
immunofixation technique, |
| キーワード |
|
|
主題 |
enzyme-linked immunoglobulin |
| キーワード |
|
|
主題 |
macroamylase, |
| キーワード |
|
|
主題 |
enzyme immunofixation |
| キーワード |
|
|
主題 |
macromolecule enzyme |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
須藤, 加代子
菅野, 剛史
嵯峨, 実枝子
堀井, 康司
加野, 象次郎
大内, 淳
|
| 書誌情報 |
生物物理化学
巻 23,
号 2,
p. 145-149,
発行日 1979-09-25
|
| 出版者 |
|
|
出版者 |
日本電気泳動学会 |
| 権利 |
|
|
権利情報 |
日本電気泳動学会 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The combination of both the electrophoresis and the immunofixation and of the thin layer gel filtration and the immunofixation followed by enzyme activity staining on antigen-antibody complexes were applied to detect and identify the enzyme-linked immunoglobulins which were observed as macromolecular enzyme complexes in human sera. For any given antigen-antibody system, the optimum concentration of antigen in the serum to be analysed must be given experimentally. Moreover, removing of unreacted proteins must be carefully performed with the solution which will protect the activity of the enzyme to be detected. In the cases of LDH- and amylase-linked immunoglobulins the detection of minimum enzyme activities on Cellogel membrane were limited by staining sensitivity. Thus, two to ten times concentration of commercially available antibodies was required to give the optimum concentration of antigenantibody complexes. In the cases of alkaline-phosphatase-linked immunoglobulins, the detection of the enzyme activity was easily amplified by the elongation of the incubation period. Thus, the optimum concentration of antigen was given without concentration of commercial antibodies. In the studies of macromolecular enzyme complexes in human sera, the immunoprecipitation technique and enzymo-immunoelectrophoresis were conventionally carried out to identify the enzyme-linked immunoglobulins. Some cases, however, still ramain ambiguous because of unskilled and unstable techniques used for analysing these macromolecular enzyme complexes. Thus, these immunofixation techniques followed by enzyme activity staining (enzyme immunofixation), described in this paper can facilitate the detection and identification of such macromolecular complexes as enzyme-linked immunoglobulins. |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00319082 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13499785 |
| 出版社DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.2198/sbk.23.145 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
Seibutubuturi23_2-145.pdf (6.4 MB)
