| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
|
|
タイトル |
Development of a Fiber-Optic Plate Microscope System and Visualization of Cells Stained with Fluorescent Dyes |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Fiber-optic plate |
| キーワード |
|
|
主題 |
video-microscopy |
| キーワード |
|
|
主題 |
fluorescence microscopy |
| キーワード |
|
|
主題 |
calcium measurement |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Hirano, Masahiko
Yamashita, Yutaka
Miyakawa, Atsuo
|
| 書誌情報 |
Analytical Sciences
巻 9,
号 3,
p. 375-380,
発行日 1993-06-10
|
| 出版者 |
|
|
出版者 |
日本分析化学会 The Japan Society for Analytical Chemistry |
| 権利 |
|
|
権利情報 |
Copyright The Japan Society for Analytical Chemistry |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
A new type of video-microscope system combined with a fiber-optic plate processed in the shape of a needle has been developed; the obtained fluorescence images were analyzed. The fiber-optic plate comprises thousands of optical fibers 3 μm in diameter, a sufficient size for viewing a single cell. Images at areas adjacent to the end of the fiber-optic plate are transmitted into an objective and detected with a video camera. They are then digitized for storage in an image processor. Using this system and fluorometric methods, cultured cells can be sufficiently visualized and any spatiotemporal change in the intracellular free calcium concentration can be measured. These specifications suggest that the activity and morphology of cells in internal parts of a living animal can be measured at the single-cell level by inserting a fiber-optic plate into the target organ. |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09106340 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13482246 |
| 出版社DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.2116/analsci.9.375 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
AnaSci9-375.pdf (522.2 kB)
