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Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure
http://hdl.handle.net/10271/2775
http://hdl.handle.net/10271/27751eb0c769-7310-4929-a0cc-34e4ef0eeac6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
論文本文 (1.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2014-10-07 | |||||||
| タイトル | ||||||||
| タイトル | Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | Imaging Mass Spectrometry (IMS) | |||||||
| キーワード | ||||||||
| 主題 | Matrix Assisted Laser Ionization/Desorption (MALDI) | |||||||
| キーワード | ||||||||
| 主題 | dental structure | |||||||
| キーワード | ||||||||
| 主題 | periodontal disease | |||||||
| キーワード | ||||||||
| 主題 | lysophosphatidylcholine (LPC) | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| その他のタイトル | ||||||||
| その他のタイトル | マトリックス支援レーザー脱離イオン化質量分析イメージング法は歯の構造と関連する歯科疾患の痕跡を明らかにした | |||||||
| 著者 |
Hirano, Hirokazu
× Hirano, Hirokazu
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| 書誌情報 |
en : Analytical and Bioanalytical Chemistry 巻 406, 号 5, p. 1355-1363, 発行日 2013 |
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| 出版者 | ||||||||
| 出版者 | Springer Berlin Heidelberg | |||||||
| 言語 | en | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Periodontal disease is a serious dental problem because it doesn’t heal naturally and leads to tooth loss. In periodontal disease, inflammation at periodontal tissue is thought as predominant, and its effect against tooth itself remains unclear. In this study, we applied matrix assisted laser desorption/ionization (MALDI) Imaging Mass Spectrometry (IMS) to teeth for the first time. By comparing anatomical structure of tooth affected with periodontal disease with normal ones, we analyzed traces of the disease on tooth. We found signals characteristic of enamel, dentin, and dental pulp, respectively, in mass spectra obtained from normal teeth. Ion images reconstructed using these signals showed anatomical structures of the tooth clearly. Next, we performed IMS upon teeth of periodontal disease. Overall characteristic of the mass spectrum appeared similar to normal ones. However, ion images reconstructed using signals from the tooth of periodontal disease revealed loss of periodontal ligament visualized together with dental pulp in normal teeth. Moreover, ion image clearly depicted an accumulation of signal at m/z 496.3 at root surface. Such an accumulation that cannot be examined only from mass spectrum was revealed by utilization of IMS. Recent studies about inflammation revealed that the signal at m/z 496.3 reflects lyso-phosphatidylcholine (LPC). Infiltration of the signal is statistically significant and its intensity profile exhibited the influence has reached deeply into the tooth. This suggests that influence of periodontal disease is not only inflammation of periodontal tissue but also infiltration of LPC to root surface, and therefore, anti-inflammatory treatment is required besides conventional treatments. (250 words) | |||||||
| 言語 | en | |||||||
| 学位名 | ||||||||
| 学位名 | 博士（医学） | |||||||
| 学位の区分 | ||||||||
| 内容記述 | doctoral | |||||||
| 学位の分野 | ||||||||
| 内容記述 | 医学系研究科 | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 13802 | |||||||
| 学位授与機関名 | 浜松医科大学 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2013-09-20 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第661号 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 1618-2642 | |||||||
| EISSN | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 1618-2650 | |||||||
| PubMed番号 | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 23727733 | |||||||
| 出版社DOI | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1007/s00216-013-7075-y | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
