| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2015-11-20 |
| タイトル |
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|
タイトル |
Determination of azide in gastric fluid and urine by flow-injection electrospray ionization tandem mass spectrometry |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Azide |
| キーワード |
|
|
主題 |
Gold |
| キーワード |
|
|
主題 |
Electrospray ionization |
| キーワード |
|
|
主題 |
Tandem mass spectrometry |
| キーワード |
|
|
主題 |
Gastric fluid |
| キーワード |
|
|
主題 |
Urine |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Minakata, Kayoko
Nozawa, Hideki
Yamagishi, Itaru
Gonmori, Kunio
Hasegawa, Koutaro
Suzuki, Masako
Watanabe, Kanako
Suzuki, Osamu
|
| 書誌情報 |
Analytical and Bioanalytical Chemistry
巻 403,
号 7,
p. 1793-1799,
発行日 2012-06
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| 出版者 |
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|
出版者 |
Springer |
| 権利 |
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|
権利情報 |
The final publication is available at Springer via http://dx.doi.org/10.1007/s00216-012-5848-3. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
A rapid method was developed to identify and quantify the azide ion (N3?) in gastric fluid and urine. N3? in diluted biological fluids was reacted with NaAuCl4 to produce Au(N3)2?, which was extracted with octanol. Five microliters of the extract were flow-injected into an electrospray ionization tandem mass spectrometric instrument. Quantification of N3? was performed by selected reaction monitoring of the product ion Au(N)(N3) ? at m/z 253 which was derived from the precursor ion Au(N3)2? at m/z 281, using 50 μL of aqueous solution within 10 min. This method was found to be linear up to 10?5 M, to have a limit of quantification of 10?7 M, a limit of detection of 3.0×10?8 M, and a coefficient of variation of ≦ 10 % at 10?7 M. In case of urine, 50 μL of urine were spiked with N3?, this was diluted to10-fold and passed through 1 mL of a resin and finally diluted to 100-fold of the original. This method was linear up to 10?3 M, had a limit of quantification of 10?5 M, a limit of detection of 3.0×10?6 M, and coefficient of variation ≦ 8.8 % for an original urine concentration of 10?5 M. The practical applicability of this method was checked by diluting 1 μL of a suspected suicide victim’s gastric fluid 20,000-fold and 1 μL of the victim’s urine 5,000-fold and then measuring the N3? levels. These levels were found to be (7.5±1.0)×10?2 M and (3.2±0.4)×10?3 M, respectively. |
| PubMed番号 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
PMID |
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関連識別子 |
22370586 |
| 出版社DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1007/s00216-012-5848-3 |
| 著者版フラグ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
abc-403-1793.pdf (5.0 MB)
