Detection of inflammatory circulating endothelial cells using human umbilical vein endothelial cells detached from culture dishes by tumor necrosis factor-alpha as control cells

Tochikubo, Junpei

doi:https://doi.org/10.4172/2329-6607.1000129

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Detection of inflammatory circulating endothelial cells using human umbilical vein endothelial cells detached from culture dishes by tumor necrosis factor-alpha as control cells

http://hdl.handle.net/10271/3133

名前 / ファイル	ライセンス	アクション
論文本文 (1.1 MB)

Item type

学位論文 / Thesis or Dissertation(1)

公開日

2016-12-27

タイトル

Detection of inflammatory circulating endothelial cells using human umbilical vein endothelial cells detached from culture dishes by tumor necrosis factor-alpha as control cells

言語

eng

キーワード

主題

Circulating endothelial cells

キーワード

主題

Human umbilical vein endothelial cells

キーワード

主題

Tumor necrosis factor-alpha

キーワード

主題

Control cells

キーワード

主題

Flow cytometry

キーワード

主題

Vascular endothelial injury

キーワード

主題

Systematic inflammatory response syndrome

キーワード

主題

Disseminated intravascular coagulation

キーワード

主題

Inte

資源タイプ

資源タイプ識別子

http://purl.org/coar/resource_type/c_db06

資源タイプ

doctoral thesis

アクセス権

open access

アクセス権URI

http://purl.org/coar/access_right/c_abf2

その他のタイトル

腫瘍壊死因子アルファによって培養皿から遊離したヒト臍帯静脈内皮細胞をコントロール細胞に用いた炎症性循環内皮細胞の検出

著者

Tochikubo, Junpei

書誌情報

en : Cardiovascular Pharmacology

巻 4, 号 1, p. 1000129, 発行日 2014-12-20

出版者

OMICS International

言語

抄録

内容記述タイプ

Abstract

内容記述

Background: Systematic inflammatory response syndrome (SIRS) with disseminated intravascular coagulation (DIC) is a severe disorder in critically ill patients and closely related to progression of vascular endothelial injury. Early diagnosis is required to prevent the progression of organ dysfunction. Circulating endothelial cells (CECs) might increase in vascular endothelial injury and play an important role in DIC diagnosis. However, the CEC detection method has not been standardized. This study aimed to establish a method for CEC detection in a critical care setting. We used human umbilical vein endothelial cells (HUVECs) detached from culture dishes by tumor necrosis factor-alpha (TNF-α) as control cells for CECs.
Methods: Cultured HUVECs were incubated in medium with TNF-α (100 ng/mL), and cells detached from culture dishes after 24 h were used as TNF-HUVECs. Cell surface molecules of normal HUVECs, TNF-HUVECs, and blood cells were analyzed using flow cytometry (FC) to search for appropriate markers for detecting CECs. Normal HUVECs and TNF-HUVECs were added to the blood and detected using FC and the immunobead method (IB) for comparing two methods. CECs were measured in healthy volunteers and intensive care unit (ICU) patients using FC.
Results: CD146 and CD105 were highly expressed in HUVECs and superior for separation of UVECs from whole blood cells. Mean detection rates of normal HUVECs were 75% in FC and 82% in IB. However, mean detection rates of TNF-HUVECs were 64% in FC and 27% in IB (p<0.05). Mean CEC counts from 20 healthy volunteers and 16 ICU patients were 2.8 cells/mL and 4.3 cells/mL, respectively. In one ICU patient with SIRS-induced DIC, CECs were elevated by 49 cells/mL.
Conclusion: CD146 and CD105 are suitable for detecting endothelial cells from blood. FC is superior to IB for detecting endothelial cells in severe inflammatory states.

言語

学位名

博士（医学）

学位の区分

内容記述

doctoral

学位の分野

内容記述

医学系研究科

学位授与機関

学位授与機関識別子Scheme

kakenhi

学位授与機関識別子

13802

学位授与機関名

浜松医科大学

学位授与年月日

2016-02-10

学位授与番号

甲第715号

ISSN

収録物識別子タイプ

PISSN

収録物識別子

2329-6607

出版社DOI

識別子タイプ

DOI

Versions

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2023-06-20 16:52:39.069722

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Detection of inflammatory circulating endothelial cells using human umbilical vein endothelial cells detached from culture dishes by tumor necrosis factor-alpha as control cells

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